Analysis of Pfmdr 1 gene in mefloquine-resistant Plasmodium falciparum.

Hye-Sook Kim, Y. Okuda, K. Begum, Y. Nagai, Y. Wataya, M. Kimura, T. Huruta

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Drug resistance in Plasmodium falciparum is a serious problem in most endemic areas. Recent studies have suggested the potential involvement of genes in the MDR gene family in resistance to quinoline-containing compounds in P. falciparum. In our present studies, a molecular analysis of pfmdr 1 in isolate strain of P. falciparum, 523a R, from Japanese mefloquine-resistant patient was done to determine the reported association of pfmdr 1 intragenic alleles and mefloquine resistance, and to examine the antimalarial activities of several antimalarial agents against the P. falciparum strain. The antimalarial activities against the strain was decreased susceptibility to mefloquine, artemisinin and halofantrine, in contrast increased susceptibility to chloroquine. The DNA sequence analysis of pfmdr 1 gene in a strain reveled no association of intragenic alleles with mefloquine resistance. Furthermore, the overexpression of pfmdr1 mRNA have been observed and it is about 7.2 times higher than sensitive strain. Our data shows that overexpression of pfmdr1 gene may be associated in mefloquine-resistance mechanism.

Original languageEnglish
Title of host publicationNucleic acids research. Supplement (2001)
Pages231-232
Number of pages2
Edition1
Publication statusPublished - 2001

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Mefloquine
Plasmodium falciparum
Antimalarials
Genes
Alleles
MDR Genes
Chloroquine
DNA Sequence Analysis
Drug Resistance
Messenger RNA

Cite this

Kim, H-S., Okuda, Y., Begum, K., Nagai, Y., Wataya, Y., Kimura, M., & Huruta, T. (2001). Analysis of Pfmdr 1 gene in mefloquine-resistant Plasmodium falciparum. In Nucleic acids research. Supplement (2001) (1 ed., pp. 231-232)

Analysis of Pfmdr 1 gene in mefloquine-resistant Plasmodium falciparum. / Kim, Hye-Sook; Okuda, Y.; Begum, K.; Nagai, Y.; Wataya, Y.; Kimura, M.; Huruta, T.

Nucleic acids research. Supplement (2001). 1. ed. 2001. p. 231-232.

Research output: Chapter in Book/Report/Conference proceedingChapter

Kim, H-S, Okuda, Y, Begum, K, Nagai, Y, Wataya, Y, Kimura, M & Huruta, T 2001, Analysis of Pfmdr 1 gene in mefloquine-resistant Plasmodium falciparum. in Nucleic acids research. Supplement (2001). 1 edn, pp. 231-232.
Kim H-S, Okuda Y, Begum K, Nagai Y, Wataya Y, Kimura M et al. Analysis of Pfmdr 1 gene in mefloquine-resistant Plasmodium falciparum. In Nucleic acids research. Supplement (2001). 1 ed. 2001. p. 231-232
Kim, Hye-Sook ; Okuda, Y. ; Begum, K. ; Nagai, Y. ; Wataya, Y. ; Kimura, M. ; Huruta, T. / Analysis of Pfmdr 1 gene in mefloquine-resistant Plasmodium falciparum. Nucleic acids research. Supplement (2001). 1. ed. 2001. pp. 231-232
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