Analysis of pathological activities of CCN proteins in bone metastasis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Bone metastasis is a common occurrence in human malignancies, including breast, prostate, and lung cancer, and is associated with a high morbidity rate because of intractable bone pain, pathological fractures, hypercalcemia, and nerve compression. Animal models of bone metastasis are important tools to investigate the pathogenesis and develop treatment strategies. However, there are few models of spontaneous bone metastasis despite the fact that animals often spontaneously develop cancer. Here, we describe methods for developing a mouse model of breast cancer bone metastasis achieved by injection of MDA-MB-231 breast cancer cells into the heart. This assay can be applied to studies on roles of CCN proteins in tumor metastasis and development of treatment strategies targeting CCN proteins.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages505-512
Number of pages8
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)1064-3745

Keywords

  • Bone
  • CCN proteins
  • CCN2
  • CTGF
  • Metastasis
  • Tumor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Shimo, T., Yoshioka, N., Takigawa, M., & Sasaki, A. (2017). Analysis of pathological activities of CCN proteins in bone metastasis. In Methods in Molecular Biology (pp. 505-512). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_42