Analysis of osteocyte morphology in terms of sensation of in vivo stress applied on bone

Takuya Ishimoto, Keita Kawahara, Aira Matsugaki, Jun Wang, Hiroshi Kamioka, Takayoshi Nakano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Most bones are anisotropically loaded and seem to be adapted to the anisotropic stress or strain field by changing the anisotropy in their microstructure. Osteocyte (OCY) is believed to play an important role as a mechanosensor and regulator of modeling and/or remodeling orchestrating osteoblast and osteoclast activity to make bone suitable to resist the mechanical environment. In general, osteocytes sense magnitude of stress (strain) applied upon the bone and then work as a trigger to change bone mass to adjust bone's mechanical function to the stress field. This structural optimization is an important aspect of the bone functional adaptation; another inevitable optimization might be achieved through the change in intrinsic material anisotropy including the preferential c-axis orientation of biological apatite (BAp) crystal. To achieve this adaptation through material anisotropy, osteocyte needs to be a mechanosensor which can detect anisotropic stress field. In the present study, osteocyte lacunae and canaliculi in the mid-diaphysis and the distal part of the rat femur were stained by a fluorescein dye for visualization and analysis. The mid-diaphysis shows greater degree of the preferential c-axis orientation of BAp crystal than the distal part in relation to the magnitude of uni-axial stress field. It was found that the osteocytes in long bone preferentially align along the bone long axis and the degree of alignment is greater in the mid-diaphysis than in the distal region, which seems to be effective for the sensation of the site-dependent specific stress field applied on the long bone.

Original languageEnglish
Title of host publicationTHERMEC 2013
PublisherTrans Tech Publications Ltd
Pages1265-1268
Number of pages4
ISBN (Print)9783038350736
Publication statusPublished - Jan 1 2014
Event8th International Conference on Processing and Manufacturing of Advanced Materials, THERMEC 2013 - Las Vegas, NV, United States
Duration: Dec 2 2013Dec 6 2013

Publication series

NameMaterials Science Forum
Volume783-786
ISSN (Print)0255-5476

Other

Other8th International Conference on Processing and Manufacturing of Advanced Materials, THERMEC 2013
CountryUnited States
CityLas Vegas, NV
Period12/2/1312/6/13

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Keywords

  • Anisotropic morphology
  • Confocal laser scanning microscopy (CLSM)
  • Fluorescent staining
  • Lacunae
  • Osteocyte
  • Stress field

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Ishimoto, T., Kawahara, K., Matsugaki, A., Wang, J., Kamioka, H., & Nakano, T. (2014). Analysis of osteocyte morphology in terms of sensation of in vivo stress applied on bone. In THERMEC 2013 (pp. 1265-1268). (Materials Science Forum; Vol. 783-786). Trans Tech Publications Ltd.