Analysis of neutral glycosphingolipids of Theileria sergenti piroplasms

Shinobu Watarai; Chihiro Sugimoto; Misao Onuma; Kazuko Kobayashi; Tatsuji Yasuda

doi:10.1292/jvms.58.165

Analysis of neutral glycosphingolipids of Theileria sergenti piroplasms

Shinobu Watarai, Chihiro Sugimoto, Misao Onuma, Kazuko Kobayashi, Tatsuji Yasuda

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Neutral glycosphingolipids (GSLs) were isolated from a lipid extract of Theileria sergenti piroplasms and analyzed by thin-layer chromatography (TLC) and liposome immune lysis assay (LILA); two predominant GSLs, designated as N-1 and N-2 were separated on TLC, N-1 GSL showed the same mobility as lactosylceramide (LacCer) on the TLC plate. On the other hand, the mobility of N-2 GSL on the TLC plate was identical to that of galactosylparagloboside. In order to characterize the molecular species of neutral GSLs from T. sergenti, N-1 and N-2 GSLs were tested by LILA with antibodies against LacCer and galactosylparagloboside, respectively. N-1 GSL had reactivity to anti-LacCer antibody and N-2 reacted with the antibody to galactosylparagloboside. These results suggest that N-1 and N-2 GSLs are LacCer (Galβ1-4Glcβ1-1Cer) and galactosylparagloboside (Galα1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer), respectively.

Original language	English
Pages (from-to)	165-168
Number of pages	4
Journal	Journal of Veterinary Medical Science
Volume	58
Issue number	2
DOIs	https://doi.org/10.1292/jvms.58.165
Publication status	Published - Feb 1996

Keywords

Glycosphingolipid
Protozoan disease
Theileria sergenti

ASJC Scopus subject areas

veterinary(all)

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10.1292/jvms.58.165

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title = "Analysis of neutral glycosphingolipids of Theileria sergenti piroplasms",

abstract = "Neutral glycosphingolipids (GSLs) were isolated from a lipid extract of Theileria sergenti piroplasms and analyzed by thin-layer chromatography (TLC) and liposome immune lysis assay (LILA); two predominant GSLs, designated as N-1 and N-2 were separated on TLC, N-1 GSL showed the same mobility as lactosylceramide (LacCer) on the TLC plate. On the other hand, the mobility of N-2 GSL on the TLC plate was identical to that of galactosylparagloboside. In order to characterize the molecular species of neutral GSLs from T. sergenti, N-1 and N-2 GSLs were tested by LILA with antibodies against LacCer and galactosylparagloboside, respectively. N-1 GSL had reactivity to anti-LacCer antibody and N-2 reacted with the antibody to galactosylparagloboside. These results suggest that N-1 and N-2 GSLs are LacCer (Galβ1-4Glcβ1-1Cer) and galactosylparagloboside (Galα1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer), respectively.",

keywords = "Glycosphingolipid, Protozoan disease, Theileria sergenti",

author = "Shinobu Watarai and Chihiro Sugimoto and Misao Onuma and Kazuko Kobayashi and Tatsuji Yasuda",

year = "1996",

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T1 - Analysis of neutral glycosphingolipids of Theileria sergenti piroplasms

AU - Watarai, Shinobu

AU - Sugimoto, Chihiro

AU - Onuma, Misao

AU - Kobayashi, Kazuko

AU - Yasuda, Tatsuji

PY - 1996/2

Y1 - 1996/2

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AB - Neutral glycosphingolipids (GSLs) were isolated from a lipid extract of Theileria sergenti piroplasms and analyzed by thin-layer chromatography (TLC) and liposome immune lysis assay (LILA); two predominant GSLs, designated as N-1 and N-2 were separated on TLC, N-1 GSL showed the same mobility as lactosylceramide (LacCer) on the TLC plate. On the other hand, the mobility of N-2 GSL on the TLC plate was identical to that of galactosylparagloboside. In order to characterize the molecular species of neutral GSLs from T. sergenti, N-1 and N-2 GSLs were tested by LILA with antibodies against LacCer and galactosylparagloboside, respectively. N-1 GSL had reactivity to anti-LacCer antibody and N-2 reacted with the antibody to galactosylparagloboside. These results suggest that N-1 and N-2 GSLs are LacCer (Galβ1-4Glcβ1-1Cer) and galactosylparagloboside (Galα1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer), respectively.

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Analysis of neutral glycosphingolipids of Theileria sergenti piroplasms

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Keywords

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