Analysis of neutral glycosphingolipids of Theileria sergenti piroplasms

Shinobu Watarai, Chihiro Sugimoto, Misao Onuma, Kazuko Kobayashi, Tatsuji Yasuda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Neutral glycosphingolipids (GSLs) were isolated from a lipid extract of Theileria sergenti piroplasms and analyzed by thin-layer chromatography (TLC) and liposome immune lysis assay (LILA); two predominant GSLs, designated as N-1 and N-2 were separated on TLC, N-1 GSL showed the same mobility as lactosylceramide (LacCer) on the TLC plate. On the other hand, the mobility of N-2 GSL on the TLC plate was identical to that of galactosylparagloboside. In order to characterize the molecular species of neutral GSLs from T. sergenti, N-1 and N-2 GSLs were tested by LILA with antibodies against LacCer and galactosylparagloboside, respectively. N-1 GSL had reactivity to anti-LacCer antibody and N-2 reacted with the antibody to galactosylparagloboside. These results suggest that N-1 and N-2 GSLs are LacCer (Galβ1-4Glcβ1-1Cer) and galactosylparagloboside (Galα1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer), respectively.

Original languageEnglish
Pages (from-to)165-168
Number of pages4
JournalJournal of Veterinary Medical Science
Volume58
Issue number2
DOIs
Publication statusPublished - Feb 1996

Keywords

  • Glycosphingolipid
  • Protozoan disease
  • Theileria sergenti

ASJC Scopus subject areas

  • veterinary(all)

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