TY - JOUR
T1 - Analysis of mRNA Using the in Situ Hybridization Technique
T2 - Diagnostic and Therapeutic Application of the in Situ Hybridization Technique
AU - Yagi, Takahito
AU - Horimi, Tadashi
AU - Okabayashi, Takahiro
AU - Tanaka, Izuru
AU - Hayashi, Doufu
AU - Miyake, Noriyuki
AU - Hirakawa, Keiichi
AU - Kagawa, Shigeo
AU - Orita, Kunzo
PY - 1988/2/1
Y1 - 1988/2/1
N2 - We used the histo in situ hybridization (HISH) technique to study the relationship between oncogene mRNA expression and survival rate and the pathological factors in gastric cancers. In excised specimens the prognosis was poor in cases those had moderate or marked c-Ha-ras mRNA expression. The degree of expression was mutually related to the degree of lymphnode metastasis. In biopsy specimens, the prognosis in the cases that expressed c-myc mRNA was also extremely poor, the 2-year survival rate being only 38.9%. The investigation of pathological factors suggested that the expression of oncogene mRNA may be a new moleculo-pathological factor in gastric cancer and an indicator of malignant potency. It appears that the pre-operative detection of oncogene mRNA in biopsy specimens can be utilized to diagnose and treat gastric cancer.
AB - We used the histo in situ hybridization (HISH) technique to study the relationship between oncogene mRNA expression and survival rate and the pathological factors in gastric cancers. In excised specimens the prognosis was poor in cases those had moderate or marked c-Ha-ras mRNA expression. The degree of expression was mutually related to the degree of lymphnode metastasis. In biopsy specimens, the prognosis in the cases that expressed c-myc mRNA was also extremely poor, the 2-year survival rate being only 38.9%. The investigation of pathological factors suggested that the expression of oncogene mRNA may be a new moleculo-pathological factor in gastric cancer and an indicator of malignant potency. It appears that the pre-operative detection of oncogene mRNA in biopsy specimens can be utilized to diagnose and treat gastric cancer.
KW - histo in situ hybridization
KW - oncogene mRNA (c-myc, c-Ha-ras)
KW - prognosis of gastric cancer
UR - http://www.scopus.com/inward/record.url?scp=85004671073&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85004671073&partnerID=8YFLogxK
U2 - 10.5833/jjgs.23.1022
DO - 10.5833/jjgs.23.1022
M3 - Article
AN - SCOPUS:85004671073
SN - 0386-9768
VL - 23
SP - 1022
EP - 1027
JO - Japanese Journal of Gastroenterological Surgery
JF - Japanese Journal of Gastroenterological Surgery
IS - 4
ER -
