Analysis of molluscum contagiosum virus genomes isolated in Japan

J. Nakamura, Y. Muraki, Masao Yamada, Y. Hatano, S. Nii

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The genomes of 477 Japanese strains of molluscum contagiosum virus (MCV) were analyzed using an in-gel digestion method with the restriction enzyme BamHI, and classified into four types, including a newly detected type (MCV type 4). All type 1 (MCV-1) genomes examined so far in Japan showed a common difference from the genome of the MCV-1 prototype (MCV-1p), the type reported to be most prevalent in Europe. The common markers of the variants of MCV-1 were 24-kbp fusion fragments generated by the loss of a BamHI site between the D2 and F fragments of MCV-1p. These variants of MCV-1 were classified into three groups (MCV-1va, MCV-1vb, MCV-1vc), with the variability among them being due to additions and losses of BamHI sites located in the right terminus and around the E and I fragments of MCV-1va. The restriction map of MCV-4 was generated and lined up with those of the other types. Cross-hybridization analysis revealed that the organization of all types of MCV genomes were essentially colinear. Considerable numbers of BamHI restriction sites were conserved between MCV-2 and 4, indicating a close analogy between them. The overall prevalence of MCV, as shown by the ratios of MCV-1 (MCV-1p):MCV-2: MCV3:MCV-4, was 436(0):13: 24:4. Thus, the molecular epidemiology of MCV in Japan is characterized by the absence of the European prototype of MCV-1, the exclusive occurrence and abundance of variants of MCV-1, a greater prevalence of MCV-3 over MCV-2, and the presence of MCV-4.

Original languageEnglish
Pages (from-to)339-348
Number of pages10
JournalJournal of Medical Virology
Volume46
Issue number4
DOIs
Publication statusPublished - 1995

Fingerprint

Molluscum contagiosum virus
Japan
Genome

Keywords

  • Epidemiology
  • Molecular
  • Molecular cloning
  • Molluscipoxvirus
  • Restriction map

ASJC Scopus subject areas

  • Virology

Cite this

Analysis of molluscum contagiosum virus genomes isolated in Japan. / Nakamura, J.; Muraki, Y.; Yamada, Masao; Hatano, Y.; Nii, S.

In: Journal of Medical Virology, Vol. 46, No. 4, 1995, p. 339-348.

Research output: Contribution to journalArticle

Nakamura, J. ; Muraki, Y. ; Yamada, Masao ; Hatano, Y. ; Nii, S. / Analysis of molluscum contagiosum virus genomes isolated in Japan. In: Journal of Medical Virology. 1995 ; Vol. 46, No. 4. pp. 339-348.
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