TY - JOUR
T1 - Analysis of interaction between liposome membranes induced by stress condition
T2 - Utilization of liposomes immobilized on indium tin oxide electrode
AU - Ishii, Haruyuki
AU - Shimanouchi, Toshinori
AU - Umakoshi, Hiroshi
AU - Kuboi, Ryoichi
N1 - Funding Information:
The authors give thanks to the Research Group of “Membrane Stress Biotechnology” and also thanks to Prof. Dr. Peter Walde (ETH-Zurich). It was supported by the Core University Program between the Japan Society for the Promotion of Science (JSPS) and Vietnam Academy of Science and Technology (VAST). It was partly supported by a Grant-in-Aid for Scientific Research (Nos. 19656203, 19656220, 20360350, 20760539, and 21246121) from the Ministry of Education, Science, Sports, and Culture of Japan, a grant from the Global COE Program “Bio-Environmental Chemistry” of JSPS. The authors are grateful to the Research Center for Solar Energy Chemistry of Osaka University and the Gas hydrate Analyzing System of Osaka University. One of the authors (H. Ishii) also deeply acknowledges the financial support of JSPS and Global COE Program.
PY - 2009/11
Y1 - 2009/11
N2 - NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.
AB - NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.
KW - Fluorescence microscope
KW - Immobilization of liposome
KW - NBD-cholesterol
KW - Stimuli responsive copolymer
KW - α-Chymotrypsin
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U2 - 10.1016/j.jbiosc.2009.05.009
DO - 10.1016/j.jbiosc.2009.05.009
M3 - Article
C2 - 19804868
AN - SCOPUS:70449417974
VL - 108
SP - 425
EP - 428
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -