Analysis of interaction between liposome membranes induced by stress condition: Utilization of liposomes immobilized on indium tin oxide electrode

Haruyuki Ishii, Toshinori Shimanouchi, Hiroshi Umakoshi, Ryoichi Kuboi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.

Original languageEnglish
Pages (from-to)425-428
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume108
Issue number5
DOIs
Publication statusPublished - Nov 2009
Externally publishedYes

Fingerprint

Liposomes
Tin oxides
Indium
Electrodes
Membranes
Cholesterol
indium tin oxide
Polymers
Chymotrypsin
Chromatography
Rate constants
Suspensions
Microscopes

Keywords

  • α-Chymotrypsin
  • Fluorescence microscope
  • Immobilization of liposome
  • NBD-cholesterol
  • Stimuli responsive copolymer

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Analysis of interaction between liposome membranes induced by stress condition : Utilization of liposomes immobilized on indium tin oxide electrode. / Ishii, Haruyuki; Shimanouchi, Toshinori; Umakoshi, Hiroshi; Kuboi, Ryoichi.

In: Journal of Bioscience and Bioengineering, Vol. 108, No. 5, 11.2009, p. 425-428.

Research output: Contribution to journalArticle

@article{36ffc94ce1ad45d88d1f3f390e0479e1,
title = "Analysis of interaction between liposome membranes induced by stress condition: Utilization of liposomes immobilized on indium tin oxide electrode",
abstract = "NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.",
keywords = "α-Chymotrypsin, Fluorescence microscope, Immobilization of liposome, NBD-cholesterol, Stimuli responsive copolymer",
author = "Haruyuki Ishii and Toshinori Shimanouchi and Hiroshi Umakoshi and Ryoichi Kuboi",
year = "2009",
month = "11",
doi = "10.1016/j.jbiosc.2009.05.009",
language = "English",
volume = "108",
pages = "425--428",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "5",

}

TY - JOUR

T1 - Analysis of interaction between liposome membranes induced by stress condition

T2 - Utilization of liposomes immobilized on indium tin oxide electrode

AU - Ishii, Haruyuki

AU - Shimanouchi, Toshinori

AU - Umakoshi, Hiroshi

AU - Kuboi, Ryoichi

PY - 2009/11

Y1 - 2009/11

N2 - NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.

AB - NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.

KW - α-Chymotrypsin

KW - Fluorescence microscope

KW - Immobilization of liposome

KW - NBD-cholesterol

KW - Stimuli responsive copolymer

UR - http://www.scopus.com/inward/record.url?scp=70449417974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70449417974&partnerID=8YFLogxK

U2 - 10.1016/j.jbiosc.2009.05.009

DO - 10.1016/j.jbiosc.2009.05.009

M3 - Article

C2 - 19804868

AN - SCOPUS:70449417974

VL - 108

SP - 425

EP - 428

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 5

ER -