Abstract
NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.
Original language | English |
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Pages (from-to) | 425-428 |
Number of pages | 4 |
Journal | Journal of Bioscience and Bioengineering |
Volume | 108 |
Issue number | 5 |
DOIs | |
Publication status | Published - Nov 1 2009 |
Externally published | Yes |
Keywords
- Fluorescence microscope
- Immobilization of liposome
- NBD-cholesterol
- Stimuli responsive copolymer
- α-Chymotrypsin
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology