Abstract
NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 425-428 |
| Number of pages | 4 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 108 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Nov 2009 |
| Externally published | Yes |
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Keywords
- α-Chymotrypsin
- Fluorescence microscope
- Immobilization of liposome
- NBD-cholesterol
- Stimuli responsive copolymer
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
Analysis of interaction between liposome membranes induced by stress condition : Utilization of liposomes immobilized on indium tin oxide electrode. / Ishii, Haruyuki; Shimanouchi, Toshinori; Umakoshi, Hiroshi; Kuboi, Ryoichi.
In: Journal of Bioscience and Bioengineering, Vol. 108, No. 5, 11.2009, p. 425-428.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of interaction between liposome membranes induced by stress condition
T2 - Utilization of liposomes immobilized on indium tin oxide electrode
AU - Ishii, Haruyuki
AU - Shimanouchi, Toshinori
AU - Umakoshi, Hiroshi
AU - Kuboi, Ryoichi
PY - 2009/11
Y1 - 2009/11
N2 - NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.
AB - NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions.
KW - α-Chymotrypsin
KW - Fluorescence microscope
KW - Immobilization of liposome
KW - NBD-cholesterol
KW - Stimuli responsive copolymer
UR - http://www.scopus.com/inward/record.url?scp=70449417974&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70449417974&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2009.05.009
DO - 10.1016/j.jbiosc.2009.05.009
M3 - Article
C2 - 19804868
AN - SCOPUS:70449417974
VL - 108
SP - 425
EP - 428
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -