Analysis of immunoglobulin a antibodies to Helicobacter pylori in serum and gastric juice in relation to mucosal inflammation

Shunji Hayashi, Toshiro Sugiyama, Kenji Yokota, Hiroshi Isogai, Emiko Isogai, Keiji Oguma, Masahiro Asaka, Nobuhiro Fujii, Yoshikazu Hirai

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Helicobacter pylori is a major etiologic agent in gastroduodenal disorders. In this study, immunoglobulin A (IgA) antibodies to H. pylori antigens were evaluated in serum and gastric juice specimens obtained from patients with gastritis or peptic ulcers by utilizing antibody capture enzyme-linked immunosorbent assays (ACELISAs). Urease α subunit (UA), urease β subunit (UB), the 66-kDa heat shock protein (HSP), and the 25-kDa protein (25K) were used as antigens for the ACELISAs. The antibody titers of the ACELISAs reflect the ratio of H. pylori-specific IgA to total IgA. The ratio is stable, although the antibody concentration fluctuates in gastric juice. By using ACELISAs it was possible to evaluate quantitatively not only serum IgA antibodies but also gastric juice secretory IgA (S-IgA) antibodies. In both serum IgA and gastric juice S-IgA ACELISAs, the titers of antibody to HSP and 25K were remarkably correlated with the histologic grade of gastritis, whereas those to UA and UB were not strongly correlated with histologic grade. Thus, it is useful for estimating the histologic grade of gastritis to quantify serum IgA and gastric juice S-IgA antibodies to HSP and 25K.

Original languageEnglish
Pages (from-to)617-621
Number of pages5
JournalClinical and Diagnostic Laboratory Immunology
Volume5
Issue number5
Publication statusPublished - 1998

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Gastric Juice
Helicobacter pylori
Immunoglobulins
Inflammation
Antibodies
Immunosorbents
Serum
Immunoglobulin A
Assays
Secretory Immunoglobulin A
Enzyme-Linked Immunosorbent Assay
Urease
Gastritis
Heat-Shock Proteins
Enzymes
Antigens
Proteins
Peptic Ulcer

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Analysis of immunoglobulin a antibodies to Helicobacter pylori in serum and gastric juice in relation to mucosal inflammation. / Hayashi, Shunji; Sugiyama, Toshiro; Yokota, Kenji; Isogai, Hiroshi; Isogai, Emiko; Oguma, Keiji; Asaka, Masahiro; Fujii, Nobuhiro; Hirai, Yoshikazu.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 5, No. 5, 1998, p. 617-621.

Research output: Contribution to journalArticle

Hayashi, S, Sugiyama, T, Yokota, K, Isogai, H, Isogai, E, Oguma, K, Asaka, M, Fujii, N & Hirai, Y 1998, 'Analysis of immunoglobulin a antibodies to Helicobacter pylori in serum and gastric juice in relation to mucosal inflammation', Clinical and Diagnostic Laboratory Immunology, vol. 5, no. 5, pp. 617-621.
Hayashi, Shunji ; Sugiyama, Toshiro ; Yokota, Kenji ; Isogai, Hiroshi ; Isogai, Emiko ; Oguma, Keiji ; Asaka, Masahiro ; Fujii, Nobuhiro ; Hirai, Yoshikazu. / Analysis of immunoglobulin a antibodies to Helicobacter pylori in serum and gastric juice in relation to mucosal inflammation. In: Clinical and Diagnostic Laboratory Immunology. 1998 ; Vol. 5, No. 5. pp. 617-621.
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