Analysis of high temperature stress on the dynamics of antenna size and reducing side heterogeneity of Photosystem II in wheat leaves (Triticum aestivum)

Sonal Mathur, Suleyman I. Allakhverdiev, Anjana Jajoo

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

This study demonstrates the effect of high temperature stress on the heterogeneous behavior of PSII in Wheat (Triticum aestivum) leaves. Photosystem II in green plant chloroplasts displays heterogeneity both in the composition of its light harvesting antenna i.e. on the basis of antenna size (α, β and γ centers) and in the ability to reduce the plastoquinone pool i.e. the reducing side of the reaction centers (QB-reducing centers and QB-non-reducing centers). Detached wheat leaves were subjected to high temperature stress of 35°C, 40°C and 45°C. The chlorophyll a (Chl a) fluorescence transient were recorded in vivo with high time resolution and analyzed according to JIP test which can quantify PS II behavior using Plant efficiency analyzer (PEA). Other than PEA, Biolyzer HP-3 software was used to evaluate different types of heterogeneity in wheat leaves. The results revealed that at high temperature, there was a change in the relative amounts of PSII α, β and γ centers. As judged from the complementary area growth curve, it seemed that with increasing temperature the PSIIα and PSIIβ centers increased at the expense of PSIIγ centers. The reducing side heterogeneity was also affected as shown by an increase in the number of QB-non-reducing centers at high temperatures. The reversibility of high temperature induced damage on PSII heterogeneity was also studied. Antenna size heterogeneity was recovered fully up to 40°C while reducing side heterogeneity showed partial recovery at 40°C. An irreversible damage to both the types of heterogeneity was observed at 45°C. The work is a significant contribution to understand the basic mechanism involved in the adaptation of crop plants to stress conditions.

Original languageEnglish
Pages (from-to)22-29
Number of pages8
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1807
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

Keywords

  • Heterogeneity
  • High temperature
  • Photosystem II
  • Stress
  • Wheat (Triticum aestivum)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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