Analysis of genomic homology of murine gammaherpesvirus (MHV)-72 to MHV-68 and impact of MHV-72 on the survival and tumorigenesis in the MHV-72-infected CB17 scid/scid and CB17+/+ mice

Wakako Oda, Jela Mistrikova, Marta Stancekova, Bernadette M. Dutia, Anthony A. Nash, Hiroyuki Takahata, Zaishun Jin, Takashi Oka, Kazuhiko Hayashi

Research output: Contribution to journalArticle

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Abstract

Murine gammaherpesvirus (MHV)-68-infected mice are well-known as models for Epstein-Barr virus (EBV)-related lymphoproliferative diseases. MHV-72 may be a relative of MHV-68, but any genetic comparison between the two (except for the M7 gene) has never been reported. The genetic compositions of MHV-72 and MHV-68 were compared and the pathology of MHV-72 infection studied in CB-17 severe combined immunodeficiency (scid/scid; SCID) and CB17 wild-type (CB17+/+) mice. The MHV-72 DNA sequence was almost identical to MHV-68 except for approximately 7000 bp corresponding to the MHV-68 M1-M3 genes. Twenty-seven of 30 MHV-72-infected SCID mice (90%) died from generalized infection with intranuclear viral inclusions for approximately 1 month, while MHV-72-infected CB17+/+ mice recovered from acute infection. Long observation and pathological study of 68 MHV-72-infected mice for up to 24 months revealed that the survival rate (29.4%) and survival time (21.3 months) of MHV-72-infected CB17+/+ mice were significantly lower (P = 0.0127) and shorter (P = 0.0065) than those of the controls (61.1% and 22.9 months), respectively. The malignancy development rate (60.3%) of the infected CB17+/+ mice was also significantly higher (P = 0.004) than those of the controls (22.2%). However, no MHV-72 DNA was detected in the tumors of infected mice. MHV-72 may have some tumor-promoting effects but the tumorigenesis in infected CB17+/+ mice is different from EBV-associated tumors.

Original languageEnglish
Pages (from-to)558-568
Number of pages11
JournalPathology International
Volume55
Issue number9
DOIs
Publication statusPublished - Sep 2005

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Carcinogenesis
Human Herpesvirus 4
Neoplasms
Infection
Intranuclear Inclusion Bodies
Severe Combined Immunodeficiency
SCID Mice
Genes
Observation
Pathology
DNA

Keywords

  • CB17 mice
  • Murine gammaherpesvirus-72
  • SCID mice
  • Tumorigenesis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Analysis of genomic homology of murine gammaherpesvirus (MHV)-72 to MHV-68 and impact of MHV-72 on the survival and tumorigenesis in the MHV-72-infected CB17 scid/scid and CB17+/+ mice. / Oda, Wakako; Mistrikova, Jela; Stancekova, Marta; Dutia, Bernadette M.; Nash, Anthony A.; Takahata, Hiroyuki; Jin, Zaishun; Oka, Takashi; Hayashi, Kazuhiko.

In: Pathology International, Vol. 55, No. 9, 09.2005, p. 558-568.

Research output: Contribution to journalArticle

Oda, Wakako ; Mistrikova, Jela ; Stancekova, Marta ; Dutia, Bernadette M. ; Nash, Anthony A. ; Takahata, Hiroyuki ; Jin, Zaishun ; Oka, Takashi ; Hayashi, Kazuhiko. / Analysis of genomic homology of murine gammaherpesvirus (MHV)-72 to MHV-68 and impact of MHV-72 on the survival and tumorigenesis in the MHV-72-infected CB17 scid/scid and CB17+/+ mice. In: Pathology International. 2005 ; Vol. 55, No. 9. pp. 558-568.
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AU - Dutia, Bernadette M.

AU - Nash, Anthony A.

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