Analysis of genomic DNA of DcACS1, a 1-aminocyclopropane-1-carboxylate synthase gene, expressed in senescing petals of carnation (Dianthus caryophyllus) and its orthologous genes in D. superbus var. longicalycinus

Taro Harada, Yuino Murakoshi, Yuka Torii, Koji Tanase, Takashi Onozaki, Shigeto Morita, Takehiro Masumura, Shigeru Satoh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Carnation (Dianthus caryophyllus) flowers exhibit climacteric ethylene production followed by petal wilting, a senescence symptom. DcACS1, which encodes 1-aminocyclopropane-1-carboxylate synthase (ACS), is a gene involved in this phenomenon. We determined the genomic DNA structure of DcACS1 by genomic PCR. In the genome of 'Light Pink Barbara', we found two distinct nucleotide sequences: one corresponding to the gene previously shown as DcACS1, designated here as DcACS1a, and the other novel one designated as DcACS1b. It was revealed that both DcACS1a and DcACS1b have five exons and four introns. These two genes had almost identical nucleotide sequences in exons, but not in some introns and 3′-UTR. Analysis of transcript accumulation revealed that DcACS1b is expressed in senescing petals as well as DcACS1a. Genomic PCR analysis of 32 carnation cultivars showed that most cultivars have only DcACS1a and some have both DcACS1a and DcACS1b. Moreover, we found two DcACS1 orthologous genes with different nucleotide sequences from D. superbus var. longicalycinus, and designated them as DsuACS1a and DsuACS1b. Petals of D. superbus var. longicalycinus produced ethylene in response to exogenous ethylene, accompanying accumulation of DsuACS1 transcripts. These data suggest that climacteric ethylene production in flowers was genetically established before the cultivation of carnation.

Original languageEnglish
Pages (from-to)519-527
Number of pages9
JournalPlant Cell Reports
Volume30
Issue number4
DOIs
Publication statusPublished - Apr 1 2011
Externally publishedYes

Keywords

  • 1-Aminocyclopropane-1-carboxylate synthase
  • Climacteric ethylene biosynthesis
  • Dianthus caryophyllus
  • Dianthus superbus var. longicalycinus
  • Petal senescence

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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