Analysis of gene expression profiles in human periodontal ligament cells under hypoxia: The protective effect of CC chemokine ligand 2 to oxygen shortage

Yukiko Kitase, Masahiko Yokozeki, Shinji Fujihara, Takashi Izawa, Shingo Kuroda, Kotaro Tanimoto, Keiji Moriyama, Eiji Tanaka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Periodontal ligament (PDL) cells appear to play important functional roles in response to mechanical stress. We hypothesized that hypoxia caused by a deformation of blood vessels and the following ischaemia may play a crucial role in differential gene expression in PDL cells affected by mechanical stress. Gene induction in cultured human PDL cells by hypoxia was analyzed using cDNA array, followed by RT-PCR analysis. Eleven hypoxia-responsive genes were found differentially expressed under low-oxygen conditions in PDL cells. Among them, CCR2, CC chemokine ligand 2 (CCL2) receptor was studied in more detail since little information is available on the role of chemokines in adaptive responses of PDL cells under hypoxia. Here we investigate whether CCR2 mediates the signalling to maintain the homeostasis of PDL cells. We found that cell death of PDL cells was induced under hypoxia with down-regulation of CCL2 mRNA expression. However, the exogenous CCL2 prevented PDL cell death under oxygen shortage with the increment of cellular inhibitor of apoptosis (cIAP) mRNA expression. The present study demonstrated substantial effects of hypoxia on gene expression of CCL2 and CCR2 in PDL cells, indicating that mechanical loading accompanied with mild hypoxia allows PDL cells to elicit adaptive responses with up-regulation of CCR2.

Original languageEnglish
Pages (from-to)618-624
Number of pages7
JournalArchives of Oral Biology
Volume54
Issue number7
DOIs
Publication statusPublished - Jul 1 2009
Externally publishedYes

Fingerprint

Cell Hypoxia
CC Chemokines
Periodontal Ligament
Transcriptome
Oxygen
Ligands
Mechanical Stress
Cell Death
CCR2 Receptors
Gene Expression
Messenger RNA
Oligonucleotide Array Sequence Analysis
Chemokines
Genes
Blood Vessels
Homeostasis
Up-Regulation
Down-Regulation
Ischemia
Hypoxia

Keywords

  • CCL2
  • CCR2
  • cDNA array analysis
  • Hypoxia
  • PDL cell

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Analysis of gene expression profiles in human periodontal ligament cells under hypoxia : The protective effect of CC chemokine ligand 2 to oxygen shortage. / Kitase, Yukiko; Yokozeki, Masahiko; Fujihara, Shinji; Izawa, Takashi; Kuroda, Shingo; Tanimoto, Kotaro; Moriyama, Keiji; Tanaka, Eiji.

In: Archives of Oral Biology, Vol. 54, No. 7, 01.07.2009, p. 618-624.

Research output: Contribution to journalArticle

Kitase, Yukiko ; Yokozeki, Masahiko ; Fujihara, Shinji ; Izawa, Takashi ; Kuroda, Shingo ; Tanimoto, Kotaro ; Moriyama, Keiji ; Tanaka, Eiji. / Analysis of gene expression profiles in human periodontal ligament cells under hypoxia : The protective effect of CC chemokine ligand 2 to oxygen shortage. In: Archives of Oral Biology. 2009 ; Vol. 54, No. 7. pp. 618-624.
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