Analysis of expression of CCN family genes in skeletal tissue-derived cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The quantitative reverse transcription polymerase chain reaction or real-time PCR has become a routine technique for the detection and comparison of amounts of specific mRNA transcripts, done by measuring amplified levels of specific cDNAs. In this chapter, we provide our real-time RT-PCR experimental procedure using SYBR Green I for the quantitative analysis of CCN family gene expression. Especially, we describe the extraction and purification steps for RNA derived from mesenchymal cells, such as chondrocytes and osteoblasts that produce a large amount of extracellular matrix in detail.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages33-41
Number of pages9
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

Keywords

  • Chondrocyte
  • Collagens
  • Extracellular matrix (ECM)
  • Matrix of bone
  • Matrix of cartilage
  • Mesenchymal cells
  • Osteoblast
  • Proteoglycans
  • qRT-PCR
  • Real-time PCR
  • RNA purification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Kawaki, H., Kubota, S., & Takigawa, M. (2017). Analysis of expression of CCN family genes in skeletal tissue-derived cells. In Methods in Molecular Biology (Vol. 1489, pp. 33-41). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_4