Analysis of expression of CCN family genes in skeletal tissue-derived cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The quantitative reverse transcription polymerase chain reaction or real-time PCR has become a routine technique for the detection and comparison of amounts of specific mRNA transcripts, done by measuring amplified levels of specific cDNAs. In this chapter, we provide our real-time RT-PCR experimental procedure using SYBR Green I for the quantitative analysis of CCN family gene expression. Especially, we describe the extraction and purification steps for RNA derived from mesenchymal cells, such as chondrocytes and osteoblasts that produce a large amount of extracellular matrix in detail.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages33-41
Number of pages9
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

Fingerprint

Real-Time Polymerase Chain Reaction
Chondrocytes
Osteoblasts
Genes
Reverse Transcription
Extracellular Matrix
Complementary DNA
RNA
Gene Expression
Polymerase Chain Reaction
Messenger RNA
SYBR Green I

Keywords

  • Chondrocyte
  • Collagens
  • Extracellular matrix (ECM)
  • Matrix of bone
  • Matrix of cartilage
  • Mesenchymal cells
  • Osteoblast
  • Proteoglycans
  • qRT-PCR
  • Real-time PCR
  • RNA purification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kawaki, H., Kubota, S., & Takigawa, M. (2017). Analysis of expression of CCN family genes in skeletal tissue-derived cells. In Methods in Molecular Biology (Vol. 1489, pp. 33-41). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_4

Analysis of expression of CCN family genes in skeletal tissue-derived cells. / Kawaki, Harumi; Kubota, Satoshi; Takigawa, Masaharu.

Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. p. 33-41 (Methods in Molecular Biology; Vol. 1489).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kawaki, H, Kubota, S & Takigawa, M 2017, Analysis of expression of CCN family genes in skeletal tissue-derived cells. in Methods in Molecular Biology. vol. 1489, Methods in Molecular Biology, vol. 1489, Humana Press Inc., pp. 33-41. https://doi.org/10.1007/978-1-4939-6430-7_4
Kawaki H, Kubota S, Takigawa M. Analysis of expression of CCN family genes in skeletal tissue-derived cells. In Methods in Molecular Biology. Vol. 1489. Humana Press Inc. 2017. p. 33-41. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6430-7_4
Kawaki, Harumi ; Kubota, Satoshi ; Takigawa, Masaharu. / Analysis of expression of CCN family genes in skeletal tissue-derived cells. Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. pp. 33-41 (Methods in Molecular Biology).
@inbook{62530529b1c84136b658f10f28f2a1a4,
title = "Analysis of expression of CCN family genes in skeletal tissue-derived cells",
abstract = "The quantitative reverse transcription polymerase chain reaction or real-time PCR has become a routine technique for the detection and comparison of amounts of specific mRNA transcripts, done by measuring amplified levels of specific cDNAs. In this chapter, we provide our real-time RT-PCR experimental procedure using SYBR Green I for the quantitative analysis of CCN family gene expression. Especially, we describe the extraction and purification steps for RNA derived from mesenchymal cells, such as chondrocytes and osteoblasts that produce a large amount of extracellular matrix in detail.",
keywords = "Chondrocyte, Collagens, Extracellular matrix (ECM), Matrix of bone, Matrix of cartilage, Mesenchymal cells, Osteoblast, Proteoglycans, qRT-PCR, Real-time PCR, RNA purification",
author = "Harumi Kawaki and Satoshi Kubota and Masaharu Takigawa",
year = "2017",
doi = "10.1007/978-1-4939-6430-7_4",
language = "English",
volume = "1489",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "33--41",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Analysis of expression of CCN family genes in skeletal tissue-derived cells

AU - Kawaki, Harumi

AU - Kubota, Satoshi

AU - Takigawa, Masaharu

PY - 2017

Y1 - 2017

N2 - The quantitative reverse transcription polymerase chain reaction or real-time PCR has become a routine technique for the detection and comparison of amounts of specific mRNA transcripts, done by measuring amplified levels of specific cDNAs. In this chapter, we provide our real-time RT-PCR experimental procedure using SYBR Green I for the quantitative analysis of CCN family gene expression. Especially, we describe the extraction and purification steps for RNA derived from mesenchymal cells, such as chondrocytes and osteoblasts that produce a large amount of extracellular matrix in detail.

AB - The quantitative reverse transcription polymerase chain reaction or real-time PCR has become a routine technique for the detection and comparison of amounts of specific mRNA transcripts, done by measuring amplified levels of specific cDNAs. In this chapter, we provide our real-time RT-PCR experimental procedure using SYBR Green I for the quantitative analysis of CCN family gene expression. Especially, we describe the extraction and purification steps for RNA derived from mesenchymal cells, such as chondrocytes and osteoblasts that produce a large amount of extracellular matrix in detail.

KW - Chondrocyte

KW - Collagens

KW - Extracellular matrix (ECM)

KW - Matrix of bone

KW - Matrix of cartilage

KW - Mesenchymal cells

KW - Osteoblast

KW - Proteoglycans

KW - qRT-PCR

KW - Real-time PCR

KW - RNA purification

UR - http://www.scopus.com/inward/record.url?scp=84991660885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991660885&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6430-7_4

DO - 10.1007/978-1-4939-6430-7_4

M3 - Chapter

C2 - 27734363

AN - SCOPUS:84991660885

VL - 1489

T3 - Methods in Molecular Biology

SP - 33

EP - 41

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -