Analysis of epitopes of mouse monoclonal antibodies against human alpha-fetoprotein

Y. Kang; E. Matsuura; T. Sakamoto; M. Sakai; Shinzo Nishi

doi:10.1159/000050624

Analysis of epitopes of mouse monoclonal antibodies against human alpha-fetoprotein

Y. Kang, E. Matsuura, T. Sakamoto, M. Sakai, Shinzo Nishi

Neutron Therapy Research Center

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Thirty-six monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFP) were analyzed for the location of their epitopes by reacting them with a set of yeast recombinant AFP proteins using ELISA. Recombinant AFP proteins containing either one, two or all three domains, i.e. domain I, domain III, domain I-II, domain II-III and domain I-II-III, were produced and secreted into the culture medium of yeast cells harboring the expression plasmids. Epitopes of 13 MAbs were localized on domain I and 17 others were on domain III. However, the exact location of the epitopes of the remaining 6 MAbs could not be defined. The epitope of an antibody, namely AFY6, which was located in domain I, was successfully mapped on an octapeptide, C¹⁷⁵KAENAVE¹⁸², using synthesized overlapping octapeptides.

Original language	English
Pages (from-to)	254-261
Number of pages	8
Journal	Tumor Biology
Volume	22
Issue number	4
DOIs	https://doi.org/10.1159/000050624
Publication status	Published - Jul 9 2001

Keywords

Alpha-fetoprotein
Epitope
Monoclonal antibody
Multipin peptide synthesis system
Yeast recombinant protein

ASJC Scopus subject areas

Cancer Research

Access to Document

10.1159/000050624

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title = "Analysis of epitopes of mouse monoclonal antibodies against human alpha-fetoprotein",

abstract = "Thirty-six monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFP) were analyzed for the location of their epitopes by reacting them with a set of yeast recombinant AFP proteins using ELISA. Recombinant AFP proteins containing either one, two or all three domains, i.e. domain I, domain III, domain I-II, domain II-III and domain I-II-III, were produced and secreted into the culture medium of yeast cells harboring the expression plasmids. Epitopes of 13 MAbs were localized on domain I and 17 others were on domain III. However, the exact location of the epitopes of the remaining 6 MAbs could not be defined. The epitope of an antibody, namely AFY6, which was located in domain I, was successfully mapped on an octapeptide, C175KAENAVE182, using synthesized overlapping octapeptides.",

keywords = "Alpha-fetoprotein, Epitope, Monoclonal antibody, Multipin peptide synthesis system, Yeast recombinant protein",

author = "Y. Kang and E. Matsuura and T. Sakamoto and M. Sakai and Shinzo Nishi",

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AU - Kang, Y.

AU - Matsuura, E.

AU - Sakamoto, T.

AU - Sakai, M.

AU - Nishi, Shinzo

PY - 2001/7/9

Y1 - 2001/7/9

N2 - Thirty-six monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFP) were analyzed for the location of their epitopes by reacting them with a set of yeast recombinant AFP proteins using ELISA. Recombinant AFP proteins containing either one, two or all three domains, i.e. domain I, domain III, domain I-II, domain II-III and domain I-II-III, were produced and secreted into the culture medium of yeast cells harboring the expression plasmids. Epitopes of 13 MAbs were localized on domain I and 17 others were on domain III. However, the exact location of the epitopes of the remaining 6 MAbs could not be defined. The epitope of an antibody, namely AFY6, which was located in domain I, was successfully mapped on an octapeptide, C175KAENAVE182, using synthesized overlapping octapeptides.

AB - Thirty-six monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFP) were analyzed for the location of their epitopes by reacting them with a set of yeast recombinant AFP proteins using ELISA. Recombinant AFP proteins containing either one, two or all three domains, i.e. domain I, domain III, domain I-II, domain II-III and domain I-II-III, were produced and secreted into the culture medium of yeast cells harboring the expression plasmids. Epitopes of 13 MAbs were localized on domain I and 17 others were on domain III. However, the exact location of the epitopes of the remaining 6 MAbs could not be defined. The epitope of an antibody, namely AFY6, which was located in domain I, was successfully mapped on an octapeptide, C175KAENAVE182, using synthesized overlapping octapeptides.

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KW - Monoclonal antibody

KW - Multipin peptide synthesis system

KW - Yeast recombinant protein

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