Analysis of epitopes of mouse monoclonal antibodies against human alpha-fetoprotein

Y. Kang; E. Matsuura; T. Sakamoto; M. Sakai; Shinzo Nishi

doi:10.1159/000050624

Analysis of epitopes of mouse monoclonal antibodies against human alpha-fetoprotein

Y. Kang, E. Matsuura, T. Sakamoto, M. Sakai, Shinzo Nishi

Neutron Therapy Research Center

Research output: Contribution to journal › Article

12 Citations (Scopus)

Abstract

Thirty-six monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFP) were analyzed for the location of their epitopes by reacting them with a set of yeast recombinant AFP proteins using ELISA. Recombinant AFP proteins containing either one, two or all three domains, i.e. domain I, domain III, domain I-II, domain II-III and domain I-II-III, were produced and secreted into the culture medium of yeast cells harboring the expression plasmids. Epitopes of 13 MAbs were localized on domain I and 17 others were on domain III. However, the exact location of the epitopes of the remaining 6 MAbs could not be defined. The epitope of an antibody, namely AFY6, which was located in domain I, was successfully mapped on an octapeptide, C¹⁷⁵KAENAVE¹⁸², using synthesized overlapping octapeptides.

Original language	English
Pages (from-to)	254-261
Number of pages	8
Journal	Tumor Biology
Volume	22
Issue number	4
DOIs	https://doi.org/10.1159/000050624
Publication status	Published - Jul 9 2001

Keywords

Alpha-fetoprotein
Epitope
Monoclonal antibody
Multipin peptide synthesis system
Yeast recombinant protein

ASJC Scopus subject areas

Cancer Research

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10.1159/000050624

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Kang, Y., Matsuura, E., Sakamoto, T., Sakai, M., & Nishi, S. (2001). Analysis of epitopes of mouse monoclonal antibodies against human alpha-fetoprotein. Tumor Biology, 22(4), 254-261. https://doi.org/10.1159/000050624

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