TY - JOUR
T1 - Analysis of epitopes of mouse monoclonal antibodies against human alpha-fetoprotein
AU - Kang, Y.
AU - Matsuura, E.
AU - Sakamoto, T.
AU - Sakai, M.
AU - Nishi, Shinzo
PY - 2001/7/9
Y1 - 2001/7/9
N2 - Thirty-six monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFP) were analyzed for the location of their epitopes by reacting them with a set of yeast recombinant AFP proteins using ELISA. Recombinant AFP proteins containing either one, two or all three domains, i.e. domain I, domain III, domain I-II, domain II-III and domain I-II-III, were produced and secreted into the culture medium of yeast cells harboring the expression plasmids. Epitopes of 13 MAbs were localized on domain I and 17 others were on domain III. However, the exact location of the epitopes of the remaining 6 MAbs could not be defined. The epitope of an antibody, namely AFY6, which was located in domain I, was successfully mapped on an octapeptide, C175KAENAVE182, using synthesized overlapping octapeptides.
AB - Thirty-six monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFP) were analyzed for the location of their epitopes by reacting them with a set of yeast recombinant AFP proteins using ELISA. Recombinant AFP proteins containing either one, two or all three domains, i.e. domain I, domain III, domain I-II, domain II-III and domain I-II-III, were produced and secreted into the culture medium of yeast cells harboring the expression plasmids. Epitopes of 13 MAbs were localized on domain I and 17 others were on domain III. However, the exact location of the epitopes of the remaining 6 MAbs could not be defined. The epitope of an antibody, namely AFY6, which was located in domain I, was successfully mapped on an octapeptide, C175KAENAVE182, using synthesized overlapping octapeptides.
KW - Alpha-fetoprotein
KW - Epitope
KW - Monoclonal antibody
KW - Multipin peptide synthesis system
KW - Yeast recombinant protein
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U2 - 10.1159/000050624
DO - 10.1159/000050624
M3 - Article
C2 - 11399951
AN - SCOPUS:0034956570
VL - 22
SP - 254
EP - 261
JO - Oncodevelopmental Biology and Medicine
JF - Oncodevelopmental Biology and Medicine
SN - 1010-4283
IS - 4
ER -
