Analysis of complete genomes of propionibacterium acnes reveals a novel plasmid and increased pseudogenes in an acne associated strain

Gabriela Kasimatis, Sorel Fitz-Gibbon, Shuta Tomida, Marthew Wong, Huiying Li

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The human skin harbors a diverse community of bacteria, including the Gram-positive, anaerobic bacterium Propionibacterium acnes. P. acnes has historically been linked to the pathogenesis of acne vulgaris, a common skin disease affecting over 80% of all adolescents in the US. To gain insight into potential P. acnes pathogenic mechanisms, we previously sequenced the complete genome of a P. acnes strain HL096PA1 that is highly associated with acne. In this study, we compared its genome to the first published complete genome KPA171202. HL096PA1 harbors a linear plasmid, pIMPLE-HL096PA1. This is the first described P. acnes plasmid. We also observed a five-fold increase of pseudogenes in HL096PA1, several of which encode proteins in carbohydrate transport and metabolism. In addition, our analysis revealed a few island-like genomic regions that are unique to HL096PA1 and a large genomic inversion spanning the ribosomal operons. Together, these findings offer a basis for understanding P. acnes virulent properties, host adaptation mechanisms, and its potential role in acne pathogenesis at the strain level. Furthermore, the plasmid identified in HL096PA1 may potentially provide a new opportunity for P. acnes genetic manipulation and targeted therapy against specific disease-associated strains.

Original languageEnglish
Article number918320
JournalBioMed Research International
Volume2013
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Propionibacterium acnes
Pseudogenes
Acne Vulgaris
Plasmids
Genes
Genome
Ports and harbors
Bacteria
Skin
Metabolism
Carbohydrates
Musculoskeletal Manipulations
Genomic Islands
Anaerobic Bacteria
Carbohydrate Metabolism
Gram-Positive Bacteria
Operon
Skin Diseases
Genetic Therapy
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Analysis of complete genomes of propionibacterium acnes reveals a novel plasmid and increased pseudogenes in an acne associated strain. / Kasimatis, Gabriela; Fitz-Gibbon, Sorel; Tomida, Shuta; Wong, Marthew; Li, Huiying.

In: BioMed Research International, Vol. 2013, 918320, 2013.

Research output: Contribution to journalArticle

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