Analysis of CCN4 function in osteogenic and osteoclastic cells using gain and loss of function approaches

Azusa Maeda, Marian Young, Mitsuaki Ono

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Analysis of CCN4 function in bone was assessed using both gain and loss of function approaches. In mice this was done by genetic engineering and homologous recombination to create mice with complete ablation of the protein. For human skeletal cells adenoviral gene transfer and shRNA were used for gain and loss of function respectively. Here we describe procedures used to make and then analyze osteogenic and osteoclastic cells with or without CCN4 to determine its role in osteogenic differentiation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages347-359
Number of pages13
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)1064-3745

Keywords

  • CCN4
  • Gene knockout
  • Osteogenic differentiation
  • Osteoprogenitor cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Maeda, A., Young, M., & Ono, M. (2017). Analysis of CCN4 function in osteogenic and osteoclastic cells using gain and loss of function approaches. In Methods in Molecular Biology (pp. 347-359). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_29