Analysis of antigenic determinants shared by two different allergens recognized by human T cells: House dust mite (Dermatophagoides pteronyssinus) and chironomid midge (Chironomus yoshimatsui)

T. Nagano, N. Ohta, M. Okano, Toshiro Ono, Y. Masuda

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9 Citations (Scopus)

Abstract

To analyse the cross-reactivity of T-cell-mediated immunity between Dermatophagoides pteronyssinus (Dp) and Chironomus yoshimatsui (Cy), the most common allergens in Japan, we established antigen-specific human T-cell lines and clones. Some but not all of the Cy-induced T-cell lines showed a significant proliferative response not only to Cy, but also to Dp. No T-cell line responded to other unrelated antigens. When we stimulated the Dp-induced T-cell clones with Cy, 3 of the 40 clones (7.5%) showed a significant proliferation, and 2 of the 3 clones produced interleukin-4 and interferon-γ, indicating their helper function. Cross-reactivity was diminished significantly after the absorption of Dp antigen in an anti-Cy affinity column. The cross-reactive epitopes were thought to be expressed on the Dp molecule of 45-53 kD. The presence of helper T cells reactive to both allergens suggests a possibility that this cross-reactivity might be involved in part in the high incidence of allergy to the 2 major allergens.

Original languageEnglish
Pages (from-to)554-559
Number of pages6
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume47
Issue number5
Publication statusPublished - 1992

Fingerprint

Dermatophagoides pteronyssinus
Chironomidae
Pyroglyphidae
Allergens
Epitopes
T-Lymphocytes
Clone Cells
Cell Line
Dermatophagoides Antigens
Antigens
Helper-Inducer T-Lymphocytes
Cellular Immunity
Interleukin-4
Interferons
Japan
Hypersensitivity
Incidence

Keywords

  • Allergens
  • Chironomid midge
  • House dust mite
  • Japan

ASJC Scopus subject areas

  • Immunology

Cite this

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abstract = "To analyse the cross-reactivity of T-cell-mediated immunity between Dermatophagoides pteronyssinus (Dp) and Chironomus yoshimatsui (Cy), the most common allergens in Japan, we established antigen-specific human T-cell lines and clones. Some but not all of the Cy-induced T-cell lines showed a significant proliferative response not only to Cy, but also to Dp. No T-cell line responded to other unrelated antigens. When we stimulated the Dp-induced T-cell clones with Cy, 3 of the 40 clones (7.5{\%}) showed a significant proliferation, and 2 of the 3 clones produced interleukin-4 and interferon-γ, indicating their helper function. Cross-reactivity was diminished significantly after the absorption of Dp antigen in an anti-Cy affinity column. The cross-reactive epitopes were thought to be expressed on the Dp molecule of 45-53 kD. The presence of helper T cells reactive to both allergens suggests a possibility that this cross-reactivity might be involved in part in the high incidence of allergy to the 2 major allergens.",
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author = "T. Nagano and N. Ohta and M. Okano and Toshiro Ono and Y. Masuda",
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T1 - Analysis of antigenic determinants shared by two different allergens recognized by human T cells

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AU - Nagano, T.

AU - Ohta, N.

AU - Okano, M.

AU - Ono, Toshiro

AU - Masuda, Y.

PY - 1992

Y1 - 1992

N2 - To analyse the cross-reactivity of T-cell-mediated immunity between Dermatophagoides pteronyssinus (Dp) and Chironomus yoshimatsui (Cy), the most common allergens in Japan, we established antigen-specific human T-cell lines and clones. Some but not all of the Cy-induced T-cell lines showed a significant proliferative response not only to Cy, but also to Dp. No T-cell line responded to other unrelated antigens. When we stimulated the Dp-induced T-cell clones with Cy, 3 of the 40 clones (7.5%) showed a significant proliferation, and 2 of the 3 clones produced interleukin-4 and interferon-γ, indicating their helper function. Cross-reactivity was diminished significantly after the absorption of Dp antigen in an anti-Cy affinity column. The cross-reactive epitopes were thought to be expressed on the Dp molecule of 45-53 kD. The presence of helper T cells reactive to both allergens suggests a possibility that this cross-reactivity might be involved in part in the high incidence of allergy to the 2 major allergens.

AB - To analyse the cross-reactivity of T-cell-mediated immunity between Dermatophagoides pteronyssinus (Dp) and Chironomus yoshimatsui (Cy), the most common allergens in Japan, we established antigen-specific human T-cell lines and clones. Some but not all of the Cy-induced T-cell lines showed a significant proliferative response not only to Cy, but also to Dp. No T-cell line responded to other unrelated antigens. When we stimulated the Dp-induced T-cell clones with Cy, 3 of the 40 clones (7.5%) showed a significant proliferation, and 2 of the 3 clones produced interleukin-4 and interferon-γ, indicating their helper function. Cross-reactivity was diminished significantly after the absorption of Dp antigen in an anti-Cy affinity column. The cross-reactive epitopes were thought to be expressed on the Dp molecule of 45-53 kD. The presence of helper T cells reactive to both allergens suggests a possibility that this cross-reactivity might be involved in part in the high incidence of allergy to the 2 major allergens.

KW - Allergens

KW - Chironomid midge

KW - House dust mite

KW - Japan

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