Analysis of amelogenin gene (AMGX, AMGY) expression in ameloblastoma

Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Pwint Han Phuu, Mehmet Gunduz, Huat Siar Chong, Shinichiro Oida, Noriyuki Nagai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Although the amelogenin gene is expressed in ameloblastoma, the precise expression pattern of X and Y amelogenin genes (AMGX, AMGY) in this tumor has not yet identified. In this study, we analyzed amelogenin gene expression in 19 samples (9 male, 10 female) of oral ameloblastomas by RT-PCR and detect the chromosomal origin of amelogenin mRNA by restriction enzyme digestion of the RT-PCR product. All tumor samples expressed amelogenin mRNA. We could detect increased level of AMGY expression in all male samples, higher than that of AMEX. It is an interesting finding as in normal male tooth development, the expression of AMGY is very much lower than that of AMGX. We postulate that epigenetic change of sex chromosomes may have some correlations with tumorigenesis of ameloblastoma. We also discuss the other possible mechanisms and points for future studies on this change in expression pattern.

Original languageEnglish
Pages (from-to)843-850
Number of pages8
JournalOral Oncology
Volume41
Issue number8
DOIs
Publication statusPublished - Sep 2005

Keywords

  • AMGX
  • AMGY
  • Ameloblastoma
  • Amelogenin
  • In situ hybridization
  • RT-PCR

ASJC Scopus subject areas

  • Oral Surgery
  • Oncology
  • Cancer Research

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