Abstract
Accumulating evidence suggests that mRNA degradation systems are crucial for various biological processes in eukaryotes. Here we provide evidence that an mRNA degradation system is associated with some plant hormones and stress responses in plants. We analysed a novel Arabidopsis abscisic acid (ABA)-hypersensitive mutant, ahg2-1, that showed ABA hypersensitivity not only in germination, but also at later developmental stages, and that displayed pleiotropic phenotypes. We found that ahg2-1 accumulated more endogenous ABA in seeds and mannitol-treated plants than did the wild type. Microarray experiments showed that the expressions of ABA-, salicylic acid- and stress-inducible genes were increased in normally grown ahg2-1 plants, suggesting that the ahg2-1 mutation somehow affects various stress responses as well as ABA responses. Map-based cloning of AHG2 revealed that this gene encodes a poly(A)-specific ribonuclease (AtPARN) that is presumed to function in mRNA degradation. Detailed analysis of the ahg2-1 mutation suggests that the mutation reduces AtPARN production. Interestingly, expression of AtPARN was induced by treatment with ABA, high salinity and osmotic stress. These results suggest that both upregulation and downregulation of gene expression by the mRNA-destabilizing activity of AtPARN are crucial for proper ABA, salicylic acid and stress responses.
| Original language | English |
|---|---|
| Pages (from-to) | 972-984 |
| Number of pages | 13 |
| Journal | Plant Journal |
| Volume | 44 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2005 |
| Externally published | Yes |
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Keywords
- Abscisic acid
- PARN
- Salicylic acid
- Stress response
ASJC Scopus subject areas
- Plant Science
Cite this
Analysis of ABA Hypersensitive Germination2 revealed the pivotal functions of PARN in stress response in Arabidopsis. / Nishimura, Noriyuki; Kitahata, Nobutaka; Seki, Motoaki; Narusaka, Yoshihiro; Narusaka, Mari; Kuromori, Takashi; Asami, Tadao; Shinozaki, Kazuo; Hirayama, Takashi.
In: Plant Journal, Vol. 44, No. 6, 12.2005, p. 972-984.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of ABA Hypersensitive Germination2 revealed the pivotal functions of PARN in stress response in Arabidopsis
AU - Nishimura, Noriyuki
AU - Kitahata, Nobutaka
AU - Seki, Motoaki
AU - Narusaka, Yoshihiro
AU - Narusaka, Mari
AU - Kuromori, Takashi
AU - Asami, Tadao
AU - Shinozaki, Kazuo
AU - Hirayama, Takashi
PY - 2005/12
Y1 - 2005/12
N2 - Accumulating evidence suggests that mRNA degradation systems are crucial for various biological processes in eukaryotes. Here we provide evidence that an mRNA degradation system is associated with some plant hormones and stress responses in plants. We analysed a novel Arabidopsis abscisic acid (ABA)-hypersensitive mutant, ahg2-1, that showed ABA hypersensitivity not only in germination, but also at later developmental stages, and that displayed pleiotropic phenotypes. We found that ahg2-1 accumulated more endogenous ABA in seeds and mannitol-treated plants than did the wild type. Microarray experiments showed that the expressions of ABA-, salicylic acid- and stress-inducible genes were increased in normally grown ahg2-1 plants, suggesting that the ahg2-1 mutation somehow affects various stress responses as well as ABA responses. Map-based cloning of AHG2 revealed that this gene encodes a poly(A)-specific ribonuclease (AtPARN) that is presumed to function in mRNA degradation. Detailed analysis of the ahg2-1 mutation suggests that the mutation reduces AtPARN production. Interestingly, expression of AtPARN was induced by treatment with ABA, high salinity and osmotic stress. These results suggest that both upregulation and downregulation of gene expression by the mRNA-destabilizing activity of AtPARN are crucial for proper ABA, salicylic acid and stress responses.
AB - Accumulating evidence suggests that mRNA degradation systems are crucial for various biological processes in eukaryotes. Here we provide evidence that an mRNA degradation system is associated with some plant hormones and stress responses in plants. We analysed a novel Arabidopsis abscisic acid (ABA)-hypersensitive mutant, ahg2-1, that showed ABA hypersensitivity not only in germination, but also at later developmental stages, and that displayed pleiotropic phenotypes. We found that ahg2-1 accumulated more endogenous ABA in seeds and mannitol-treated plants than did the wild type. Microarray experiments showed that the expressions of ABA-, salicylic acid- and stress-inducible genes were increased in normally grown ahg2-1 plants, suggesting that the ahg2-1 mutation somehow affects various stress responses as well as ABA responses. Map-based cloning of AHG2 revealed that this gene encodes a poly(A)-specific ribonuclease (AtPARN) that is presumed to function in mRNA degradation. Detailed analysis of the ahg2-1 mutation suggests that the mutation reduces AtPARN production. Interestingly, expression of AtPARN was induced by treatment with ABA, high salinity and osmotic stress. These results suggest that both upregulation and downregulation of gene expression by the mRNA-destabilizing activity of AtPARN are crucial for proper ABA, salicylic acid and stress responses.
KW - Abscisic acid
KW - PARN
KW - Salicylic acid
KW - Stress response
UR - http://www.scopus.com/inward/record.url?scp=31144479273&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31144479273&partnerID=8YFLogxK
U2 - 10.1111/j.1365-313X.2005.02589.x
DO - 10.1111/j.1365-313X.2005.02589.x
M3 - Article
C2 - 16359390
AN - SCOPUS:31144479273
VL - 44
SP - 972
EP - 984
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 6
ER -