TY - JOUR
T1 - Anabolic utilization of steroid hormones in Helicobacter pylori
AU - Hosoda, Kouichi
AU - Shimomura, Hirofumi
AU - Hayashi, Shunji
AU - Yokota, Kenji
AU - Oguma, Keiji
AU - Hirai, Yoshikazu
PY - 2009/8
Y1 - 2009/8
N2 - In this study, we have demonstrated that Helicobacter pylori absorbs a steroid prehormone (pregnenolone) and two androgens (dehydroepiandrosterone and epiandrosterone), glucosylates these steroids, and utilizes glucosyl-steroid hormone compounds as the membrane lipid components. The only common structure among the steroid prehormone and the two androgens is a 3β-OH in the steroid framework. Our results indicate that the 3β-OH in the steroid hormones is a crucial conformation required for steroid glucosylation by H. pylori. In addition, we found that H. pylori absorbs and holds estrogens possessing 3-OH (estrone and estradiol) into the membrane. The effective absorption of estrogen into the membrane appeared to be controlled by the number of hydroxyl groups modifying the steroid framework. In contrast, H. pylori induced neither membrane absorption nor glucosylation of the other steroid hormones possessing 3=O (progesterone, androstenedione and testosterone) or 3α-OH (androsterone). These results indicate that H. pylori selectively absorbs 3β-OH and 3-OH steroid hormones, and utilizes only 3β-OH steroid hormones as the materials for glucosylation.
AB - In this study, we have demonstrated that Helicobacter pylori absorbs a steroid prehormone (pregnenolone) and two androgens (dehydroepiandrosterone and epiandrosterone), glucosylates these steroids, and utilizes glucosyl-steroid hormone compounds as the membrane lipid components. The only common structure among the steroid prehormone and the two androgens is a 3β-OH in the steroid framework. Our results indicate that the 3β-OH in the steroid hormones is a crucial conformation required for steroid glucosylation by H. pylori. In addition, we found that H. pylori absorbs and holds estrogens possessing 3-OH (estrone and estradiol) into the membrane. The effective absorption of estrogen into the membrane appeared to be controlled by the number of hydroxyl groups modifying the steroid framework. In contrast, H. pylori induced neither membrane absorption nor glucosylation of the other steroid hormones possessing 3=O (progesterone, androstenedione and testosterone) or 3α-OH (androsterone). These results indicate that H. pylori selectively absorbs 3β-OH and 3-OH steroid hormones, and utilizes only 3β-OH steroid hormones as the materials for glucosylation.
KW - Glucosylation
KW - Helicobacter pylori
KW - Steroid hormone
UR - http://www.scopus.com/inward/record.url?scp=68249152150&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68249152150&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2009.01685.x
DO - 10.1111/j.1574-6968.2009.01685.x
M3 - Article
C2 - 19566683
AN - SCOPUS:68249152150
VL - 297
SP - 173
EP - 179
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -