An upstream activating sequence containing curved DNA involved in activation of the Clostridium perfringens plc promoter

Chieko Matsushita, Osamu Matsushita, Seiichi Katayama, Junzaburo Minami, Kenichi Takai, Akinobu Okabe

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The plc gene, which encodes phospholipase C (α-toxin) of Clostridium perfringens, possesses three poly(A) tracts forming an intrinsically curved DNA region immediately upstream of the promoter. The in vivo transcriptional activity of the plasmid-borne plc gene was stimulated by this curved-DNA-containing sequence, depending on its proper linear and rotational orientation. The in vitro transcriptional activity of the plc gene was also stimulated by the upstream sequence. In addition, the stimulatory effect of the sequence and the degree of DNA bending were greater at lower temperature, as was demonstrated by both in vitro and in vivo transcription assays, and a gel-mobility assay, respectively. A similar temperature effect was also observed with the chromosomal plc gene. These observations suggest that the upstream DNA curvature per se stimulates the initiation of transcription of the plc gene, possibly through direct contact with RNA polymerase.

Original languageEnglish
Pages (from-to)2561-2566
Number of pages6
JournalMicrobiology
Volume142
Issue number9
DOIs
Publication statusPublished - Sep 1996

Keywords

  • Clostridium perfringens
  • Curved DNA
  • Gene expression
  • Phospholipase C

ASJC Scopus subject areas

  • Microbiology

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