An RND-type multidrug efflux pump SdeXY from Serratia marcescens

Jing Chen, Teruo Kuroda, Md Nazmul Huda, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Objectives: Serratia marcescens, an important cause of nosocomial infections, shows intrinsic resistance to a wide variety of antimicrobial agents (multidrug resistance). Multidrug efflux pumps are often involved in the multidrug resistance in many bacteria. A study was undertaken to characterize the multidrug efflux pumps in S. marcescens. Methods: The genes responsible for the multidrug resistance phenotype in S. marcescens were cloned into Escherichia coli KAM32, a drug-hypersusceptible strain, for further analysis. Results: We cloned sdeXY genes and determined the nucleotide sequence. Clones that carried the sdeXY genes displayed reduced susceptibility to several antimicrobial agents including erythromycin, tetracycline, norfloxacin, benzalkonium chloride, ethidium bromide, acriflavine and rhodamine 6G. A protein similarity search using GenBank revealed that SdeY is a member of the resistance nodulation cell-division (RND) family of multidrug efflux proteins and SdeX is a member of the membrane fusion proteins. Introduction of sdeXY into E. coli cells possessing tolC, but not in cells lacking tolC, resulted in multidrug resistance. We observed energy-dependent ethidium efflux in cells of E. coliKAM32 possessing sdeXY and tolC. Conclusions: SdeXY is the first RND-type multidrug efflux pump to be characterized in multidrug-resistant S. marcescens.

Original languageEnglish
Pages (from-to)176-179
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume52
Issue number2
DOIs
Publication statusPublished - Aug 1 2003

Fingerprint

Serratia marcescens
Cell Division
Multiple Drug Resistance
Ethidium
Anti-Infective Agents
Membrane Fusion Proteins
Acriflavine
Escherichia coli
Benzalkonium Compounds
MDR Genes
Norfloxacin
Nucleic Acid Databases
Erythromycin
Cross Infection
Tetracycline
Genes
Proteins
Clone Cells
Bacteria
Phenotype

Keywords

  • Multidrug efflux pump
  • RND family
  • S. marcescens
  • SdeXY

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology

Cite this

Chen, J., Kuroda, T., Huda, M. N., Mizushima, T., & Tsuchiya, T. (2003). An RND-type multidrug efflux pump SdeXY from Serratia marcescens. Journal of Antimicrobial Chemotherapy, 52(2), 176-179. https://doi.org/10.1093/jac/dkg308

An RND-type multidrug efflux pump SdeXY from Serratia marcescens. / Chen, Jing; Kuroda, Teruo; Huda, Md Nazmul; Mizushima, Tohru; Tsuchiya, Tomofusa.

In: Journal of Antimicrobial Chemotherapy, Vol. 52, No. 2, 01.08.2003, p. 176-179.

Research output: Contribution to journalArticle

Chen, J, Kuroda, T, Huda, MN, Mizushima, T & Tsuchiya, T 2003, 'An RND-type multidrug efflux pump SdeXY from Serratia marcescens', Journal of Antimicrobial Chemotherapy, vol. 52, no. 2, pp. 176-179. https://doi.org/10.1093/jac/dkg308
Chen, Jing ; Kuroda, Teruo ; Huda, Md Nazmul ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / An RND-type multidrug efflux pump SdeXY from Serratia marcescens. In: Journal of Antimicrobial Chemotherapy. 2003 ; Vol. 52, No. 2. pp. 176-179.
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