An organic-inorganic hybrid scaffold for the culture of HepG2 cells in a bioreactor

Ken Kataoka, Yoshitaka Nagao, Takamasa Nukui, Ichiro Akiyama, Kanji Tsuru, Satoshi Hayakawa, Akiyoshi Osaka, Nam Ho Huh

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Much interest has recently been shown in the potential utility of bioartificial liver (BAL) as a bridge support for patients and as a module for experimental purposes. A radial-flow bioreactor (RFB), one of the perfused bed/scaffold-type bioreactors, enables a highly functional three-dimensional culture as BAL. The functional capacity of bioreactors depends not only on their mechanistic structures but also on scaffolds packed in them. In the present study, we examined the possible utility of a new porous organic-inorganic-hybrid scaffold in an RFB. The scaffold was made from tetraethoxysilane (TEOS) and polydimethylsiloxane (PDMS) by a sol-gel method using sieved sucrose particles as a porogen. In the porous TEOS-PDMS hybrid scaffold, human hepatocellular carcinoma cells (HepG2) proliferated actively and formed cell clusters more efficiently than they did in a polyvinyl-alcohol scaffold. When cultivated in PDMS-TEOS, HepG2 cells secreted a ∼three-fold greater amount of albumin than that secreted in a monolayer culture. For potential application of BAL to pharmacological studies and future clinical use, it is essential to develop a method to propagate liver cells that maintain highly specific functions. The present results indicate that PDMS-TEOS may be a promising scaffold for developing such functional culture methods.

Original languageEnglish
Pages (from-to)2509-2516
Number of pages8
JournalBiomaterials
Volume26
Issue number15
DOIs
Publication statusPublished - May 2005

Fingerprint

Hep G2 Cells
Bioreactors
Artificial Liver
Scaffolds
Polydimethylsiloxane
Liver
Radial flow
Polyvinyl Alcohol
Polymers
Polymethyl Methacrylate
Sucrose
Albumins
Hepatocellular Carcinoma
Gels
Pharmacology
Polyvinyl alcohols
Sugar (sucrose)
baysilon
tetraethoxysilane
Sol-gel process

Keywords

  • Bioreactor
  • HepG2
  • Organic-inorganic hybrid
  • Scaffold
  • TEOS-PDMS
  • Three-dimensional culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

An organic-inorganic hybrid scaffold for the culture of HepG2 cells in a bioreactor. / Kataoka, Ken; Nagao, Yoshitaka; Nukui, Takamasa; Akiyama, Ichiro; Tsuru, Kanji; Hayakawa, Satoshi; Osaka, Akiyoshi; Huh, Nam Ho.

In: Biomaterials, Vol. 26, No. 15, 05.2005, p. 2509-2516.

Research output: Contribution to journalArticle

Kataoka, K, Nagao, Y, Nukui, T, Akiyama, I, Tsuru, K, Hayakawa, S, Osaka, A & Huh, NH 2005, 'An organic-inorganic hybrid scaffold for the culture of HepG2 cells in a bioreactor', Biomaterials, vol. 26, no. 15, pp. 2509-2516. https://doi.org/10.1016/j.biomaterials.2004.07.012
Kataoka, Ken ; Nagao, Yoshitaka ; Nukui, Takamasa ; Akiyama, Ichiro ; Tsuru, Kanji ; Hayakawa, Satoshi ; Osaka, Akiyoshi ; Huh, Nam Ho. / An organic-inorganic hybrid scaffold for the culture of HepG2 cells in a bioreactor. In: Biomaterials. 2005 ; Vol. 26, No. 15. pp. 2509-2516.
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