An isolate of Alternaria alternata that is pathogenic to both tangerines and rough lemon and produces two host-selective toxins, ACT- and ACR-toxins

A. Masunaka, K. Ohtani, T. L. Peever, L. W. Timmer, T. Tsuge, Mikihiro Yamamoto, H. Yamamoto, K. Akimitsu

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Two different pathotypes of Alternaria alternata cause Alternaria brown spot of tangerines and Alternaria leaf spot of rough lemon. The former produces the host-selective ACT-toxin and the latter produces ACR-toxin. Both pathogens induce similar symptoms on leaves or young fruits of their respective hosts, but the host ranges of these pathogens are distinct and one pathogen can be easily distinguished from another by comparing host ranges. We isolated strain BC3-5-1-OS2A from a leaf spot on rough lemon in Florida, and this isolate is pathogenic on both cv. Iyokan tangor and rough lemon and also produces both ACT-toxin and ACR-toxin. Isolate BC3-5-1-OS2A carries both genomic regions, one of which was known only to be present in ACT-toxin producers and the other was known to exist only in ACR-toxin producers. Each of the genomic regions is present on distinct small chromosomes, one of 1.05 Mb and the other of 2.0 Mb. Alternaria species have no known sexual or parasexual cycle in nature and populations of A. alternata on citrus are clonal. Therefore, the ability to produce both toxins was not likely acquired through meiotic or mitotic recombination. We hypothesize that a dispensable chromosome carrying the gene cluster controlling biosynthesis of one of the host-selective toxins was transferred horizontally and rearranged by duplication or translocation in another isolate of the fungus carrying genes for biosynthesis of the other host-selective toxin.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalPhytopathology
Volume95
Issue number3
DOIs
Publication statusPublished - Mar 2005

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Citrus jambhiri
tangerines
Alternaria alternata
toxins
Alternaria
leaf spot
host range
pathogens
parasexual cycle
mitotic recombination
biosynthesis
chromosomes
genomics
pathotypes
multigene family
signs and symptoms (plants)
Citrus

Keywords

  • Anastomosis

ASJC Scopus subject areas

  • Plant Science

Cite this

An isolate of Alternaria alternata that is pathogenic to both tangerines and rough lemon and produces two host-selective toxins, ACT- and ACR-toxins. / Masunaka, A.; Ohtani, K.; Peever, T. L.; Timmer, L. W.; Tsuge, T.; Yamamoto, Mikihiro; Yamamoto, H.; Akimitsu, K.

In: Phytopathology, Vol. 95, No. 3, 03.2005, p. 241-247.

Research output: Contribution to journalArticle

Masunaka, A. ; Ohtani, K. ; Peever, T. L. ; Timmer, L. W. ; Tsuge, T. ; Yamamoto, Mikihiro ; Yamamoto, H. ; Akimitsu, K. / An isolate of Alternaria alternata that is pathogenic to both tangerines and rough lemon and produces two host-selective toxins, ACT- and ACR-toxins. In: Phytopathology. 2005 ; Vol. 95, No. 3. pp. 241-247.
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