An iron-regulated gene required for utilization of aerobactin as an exogenous siderophore in Vibrio parahaemolyticus

Tatsuya Funahashi, Tomotaka Tanabe, Hiroaki Aso, Hiroshi Nakao, Yoshio Fujii, Keinosuke Okamoto, Shizuo Narimatsu, Shigeo Yamamoto

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A previous investigation using the Fur titration assay system showed that Vibrio parahaemolyticus possesses a gene encoding a protein homologous to IutA, the outer-membrane receptor for ferric aerobactin in Escherichia coli. In this study, a 5.6 kb DNA region from the V. parahaemolyticus WP1 genome was cloned and two entire genes, iutA and alcD homologues, were identified which are absent from Vibrio cholerae genomic sequences. The V. parahaemolyticus IutA and AlcD proteins share 43 % identity with the Escherichia coli IutA protein and 24 % identity with the Bordetella bronchiseptica AlcD protein of unknown function, respectively. Primer extension analysis revealed that the iutA gene is transcribed in response to low-iron availability from a putative promoter overlapped with a sequence resembling a consensus E. coli Fur-binding sequence. In agreement with the above finding, V. parahaemolyticus effectively utilized exogenously supplied aerobactin for growth under iron-limiting conditions. Moreover, insertional inactivation of iutA impaired growth in the presence of aerobactin and incapacitated the outer-membrane fraction from iron-deficient cells for binding 55Fe-labelled aerobactin. These results indicate that the V. parahaemolyticus iutA homologue encodes an outer-membrane protein which functions as the receptor for ferric aerobactin. Southern blot analysis revealed that the iutA homologues are widely distributed in clinical and environmental isolates of V. parahaemolyticus. However, additional genes required for ferric aerobactin transport across the inner membrane remain to be clarified.

Original languageEnglish
Pages (from-to)1217-1225
Number of pages9
JournalMicrobiology
Volume149
Issue number5
DOIs
Publication statusPublished - May 1 2003

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Vibrio parahaemolyticus
Siderophores
Iron
Genes
Membranes
Bordetella bronchiseptica
Escherichia coli
Proteins
Vibrio cholerae
Escherichia coli Proteins
Growth
Southern Blotting
aerobactin
Membrane Proteins
Genome
DNA

ASJC Scopus subject areas

  • Microbiology

Cite this

An iron-regulated gene required for utilization of aerobactin as an exogenous siderophore in Vibrio parahaemolyticus. / Funahashi, Tatsuya; Tanabe, Tomotaka; Aso, Hiroaki; Nakao, Hiroshi; Fujii, Yoshio; Okamoto, Keinosuke; Narimatsu, Shizuo; Yamamoto, Shigeo.

In: Microbiology, Vol. 149, No. 5, 01.05.2003, p. 1217-1225.

Research output: Contribution to journalArticle

Funahashi, T, Tanabe, T, Aso, H, Nakao, H, Fujii, Y, Okamoto, K, Narimatsu, S & Yamamoto, S 2003, 'An iron-regulated gene required for utilization of aerobactin as an exogenous siderophore in Vibrio parahaemolyticus', Microbiology, vol. 149, no. 5, pp. 1217-1225. https://doi.org/10.1099/mic.0.26066-0
Funahashi, Tatsuya ; Tanabe, Tomotaka ; Aso, Hiroaki ; Nakao, Hiroshi ; Fujii, Yoshio ; Okamoto, Keinosuke ; Narimatsu, Shizuo ; Yamamoto, Shigeo. / An iron-regulated gene required for utilization of aerobactin as an exogenous siderophore in Vibrio parahaemolyticus. In: Microbiology. 2003 ; Vol. 149, No. 5. pp. 1217-1225.
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