An intracellular mechanism of aluminum tolerance associated with high antioxidant status in cultured tobacco cells

S. Rama Devi, Yoko Yamamoto, Hideaki Matsumoto

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

An aluminum (Al) tolerance mechanism, together with oxidative stress tolerance, was investigated in an Al tolerant cell line (ALT301) and the parental Al sensitive cell line (SL) of tobacco. During Al exposure in a simple calcium solution for 24 h, Al triggered the evolution of a reactive oxygen species (ROS) in SL much higher than ALT301 [Plant Physiol. 128 (2002) 63]. Under the conditions, Al enhanced comparable rates of citrate secretion from both cell lines to the same extent. Al enhanced the gene expression of manganese superoxide dismutase (MnSOD) in both cell lines, but at a significantly higher rate in SL than in ALT301, and also enhanced the enzyme activity of MnSOD in both cell lines to nearly the same level. These results suggest that the extracellular chelation of Al with organic acids and MnSOD is not involved in the mechanism of Al tolerance of ALT301. ALT301 contained ascorbate (ASA) and glutathione (GSH) levels that were higher than SL under normal growth conditions. During 24 h of post-Al treatment culture in growth medium, but not during 24-h Al exposure in a simple Ca2+ solution, lipid peroxidation was enhanced in SL much higher than in ALT301, and the average SL amounts of ASA and GSH were exhausted compared to ALT301. Pre-loading of ASA prior to Al treatment improved the growth of SL during the post-Al treatment culture. ALT301 also exhibited cross-tolerance to H 2O2, Fe2+ and Cu2+. Under these oxidant exposures, ALT301 contained lower levels of intracellular H 2O2 or lipid peroxides, and maintained higher amounts of ASA and GSH than SL. Taken together, we conclude that the accumulation of Al in cells enhances the peroxidation of lipids exclusively under growing conditions, and that the higher content of ASA and GSH in ALT301 than in SL seems to be in part responsible for the tolerance mechanism of ALT301 to Al by protecting cells from either lipid peroxidation or H2O2 commonly enhanced by Al or other oxidants.

Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalJournal of Inorganic Biochemistry
Volume97
Issue number1
DOIs
Publication statusPublished - Sep 15 2003

Fingerprint

Tobacco
Aluminum
Cultured Cells
Antioxidants
Cell Line
Cells
Lipid Peroxidation
Superoxide Dismutase
Lipids
Oxidants
Growth
Oxidative stress
Lipid Peroxides
Organic acids
Enzyme activity
Chelation
Cell culture
Gene expression
Citric Acid

Keywords

  • Aluminum tolerance
  • Ascorbic acid
  • Glutathione
  • Oxidative stress
  • Tobacco cells

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

An intracellular mechanism of aluminum tolerance associated with high antioxidant status in cultured tobacco cells. / Devi, S. Rama; Yamamoto, Yoko; Matsumoto, Hideaki.

In: Journal of Inorganic Biochemistry, Vol. 97, No. 1, 15.09.2003, p. 59-68.

Research output: Contribution to journalArticle

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