Abstract
We developed a novel method for the detection of Mycoplasma hominis from vaginal swabs using an indirect immunofluorescence technique. It is a rapid and simple method that can be finished in only 5 hr and is more sensitive than the usual culture isolation method. The indirect immunofluorescence method was applied to vaginal smears from 193 healthy women and 33.7% gave a positive test. This value was much higher than that (11.4%) obtained from the same specimens by the culture method. When vaginal smears were subjected to Papanicolaou staining after the indirect immunofluorescence method, the specific immunofluorescence of the epithelial cells was located exactly at the sites of granular aggregates stained with Papanicolaou stain. A histological examination by Papanicolaou staining showed that the incidence of inflammation seems to be slightly higher in M. hominis carriers than in non-carriers.
| Original language | English |
|---|---|
| Pages (from-to) | 831-839 |
| Number of pages | 9 |
| Journal | Microbiology and Immunology |
| Volume | 35 |
| Issue number | 10 |
| Publication status | Published - 1991 |
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ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Microbiology (medical)
Cite this
An indirect immunofluorescence method for detection of Mycoplasma hominis in vaginal smears. / Hirai, Y.; Kanatani, T.; Matsushita, Osamu; Matsushita, O.; Kanemasa, Y.
In: Microbiology and Immunology, Vol. 35, No. 10, 1991, p. 831-839.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An indirect immunofluorescence method for detection of Mycoplasma hominis in vaginal smears
AU - Hirai, Y.
AU - Kanatani, T.
AU - Matsushita, Osamu
AU - Matsushita, O.
AU - Kanemasa, Y.
PY - 1991
Y1 - 1991
N2 - We developed a novel method for the detection of Mycoplasma hominis from vaginal swabs using an indirect immunofluorescence technique. It is a rapid and simple method that can be finished in only 5 hr and is more sensitive than the usual culture isolation method. The indirect immunofluorescence method was applied to vaginal smears from 193 healthy women and 33.7% gave a positive test. This value was much higher than that (11.4%) obtained from the same specimens by the culture method. When vaginal smears were subjected to Papanicolaou staining after the indirect immunofluorescence method, the specific immunofluorescence of the epithelial cells was located exactly at the sites of granular aggregates stained with Papanicolaou stain. A histological examination by Papanicolaou staining showed that the incidence of inflammation seems to be slightly higher in M. hominis carriers than in non-carriers.
AB - We developed a novel method for the detection of Mycoplasma hominis from vaginal swabs using an indirect immunofluorescence technique. It is a rapid and simple method that can be finished in only 5 hr and is more sensitive than the usual culture isolation method. The indirect immunofluorescence method was applied to vaginal smears from 193 healthy women and 33.7% gave a positive test. This value was much higher than that (11.4%) obtained from the same specimens by the culture method. When vaginal smears were subjected to Papanicolaou staining after the indirect immunofluorescence method, the specific immunofluorescence of the epithelial cells was located exactly at the sites of granular aggregates stained with Papanicolaou stain. A histological examination by Papanicolaou staining showed that the incidence of inflammation seems to be slightly higher in M. hominis carriers than in non-carriers.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025933631&partnerID=8YFLogxK
M3 - Article
C2 - 1779888
AN - SCOPUS:0025933631
VL - 35
SP - 831
EP - 839
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 10
ER -