An indirect immunofluorescence method for detection of Mycoplasma hominis in vaginal smears

Y. Hirai, T. Kanatani, Osamu Matsushita, O. Matsushita, Y. Kanemasa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We developed a novel method for the detection of Mycoplasma hominis from vaginal swabs using an indirect immunofluorescence technique. It is a rapid and simple method that can be finished in only 5 hr and is more sensitive than the usual culture isolation method. The indirect immunofluorescence method was applied to vaginal smears from 193 healthy women and 33.7% gave a positive test. This value was much higher than that (11.4%) obtained from the same specimens by the culture method. When vaginal smears were subjected to Papanicolaou staining after the indirect immunofluorescence method, the specific immunofluorescence of the epithelial cells was located exactly at the sites of granular aggregates stained with Papanicolaou stain. A histological examination by Papanicolaou staining showed that the incidence of inflammation seems to be slightly higher in M. hominis carriers than in non-carriers.

Original languageEnglish
Pages (from-to)831-839
Number of pages9
JournalMicrobiology and Immunology
Volume35
Issue number10
Publication statusPublished - 1991

Fingerprint

Mycoplasma hominis
Vaginal Smears
Indirect Fluorescent Antibody Technique
Staining and Labeling
Fluorescent Antibody Technique
Coloring Agents
Epithelial Cells
Inflammation
Incidence

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology
  • Microbiology (medical)

Cite this

An indirect immunofluorescence method for detection of Mycoplasma hominis in vaginal smears. / Hirai, Y.; Kanatani, T.; Matsushita, Osamu; Matsushita, O.; Kanemasa, Y.

In: Microbiology and Immunology, Vol. 35, No. 10, 1991, p. 831-839.

Research output: Contribution to journalArticle

Hirai, Y, Kanatani, T, Matsushita, O, Matsushita, O & Kanemasa, Y 1991, 'An indirect immunofluorescence method for detection of Mycoplasma hominis in vaginal smears', Microbiology and Immunology, vol. 35, no. 10, pp. 831-839.
Hirai, Y. ; Kanatani, T. ; Matsushita, Osamu ; Matsushita, O. ; Kanemasa, Y. / An indirect immunofluorescence method for detection of Mycoplasma hominis in vaginal smears. In: Microbiology and Immunology. 1991 ; Vol. 35, No. 10. pp. 831-839.
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