TY - JOUR
T1 - An Indirect Immunofluorescence Method for Detection of Mycoplasma hominis in Vaginal Smears
AU - Hirai, Yoshikazu
AU - Kanatani, Tomohisa
AU - Matsushita, Osamu
AU - Kanemasa, Yasuhiro
AU - Ono, Masako
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1991
Y1 - 1991
N2 - We developed a novel method for the detection of Mycoplasma hominis from vaginal swabs using an indirect immunofluorescence technique. It is a rapid and simple method that can be finished in only 5 hr and is more sensitive than the usual culture isolation method. The indirect immunofluorescence method was applied to vaginal smears from 193 healthy women and 33.7% gave a positive test. This value was much higher than that (11.4%) obtained from the same specimens by the culture method. When vaginal smears were subjected to Papanicolaou staining after the indirect immunofluorescence method, the specific immunofluorescence of the epithelial cells was located exactly at the sites of granular aggregates stained with Papanicolaou stain. A histological examination by Papanicolaou staining showed that the incidence of inflammation seems to be slightly higher in M. hominis-carriers than in non-carriers.
AB - We developed a novel method for the detection of Mycoplasma hominis from vaginal swabs using an indirect immunofluorescence technique. It is a rapid and simple method that can be finished in only 5 hr and is more sensitive than the usual culture isolation method. The indirect immunofluorescence method was applied to vaginal smears from 193 healthy women and 33.7% gave a positive test. This value was much higher than that (11.4%) obtained from the same specimens by the culture method. When vaginal smears were subjected to Papanicolaou staining after the indirect immunofluorescence method, the specific immunofluorescence of the epithelial cells was located exactly at the sites of granular aggregates stained with Papanicolaou stain. A histological examination by Papanicolaou staining showed that the incidence of inflammation seems to be slightly higher in M. hominis-carriers than in non-carriers.
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U2 - 10.1111/j.1348-0421.1991.tb02023.x
DO - 10.1111/j.1348-0421.1991.tb02023.x
M3 - Article
C2 - 1779888
AN - SCOPUS:0025933631
VL - 35
SP - 831
EP - 839
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 10
ER -