An in vitro screening method for DNA cytosine-C5-Methylase inhibitor

Takashi Tamura, Atsushi Kataoka, Li Yin Shu, Akihiko Ashida, Hidehiko Tanaka, Kenji Inagaki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A specific inhibitor of DNA cytosine C5-methylases would be useful for studying genomic imprinting, X-chromosome inactivation, carcinogenesis, and regulation of tissue-specific gene expression, for these physiological phenomena appears to be regulated through DNA methylation in promoter sequences. This paper reports a novel convenient in vitro assay method for screening DNA cytosine C5-methylase inhibitor. Our method uses a commercially available Hae III methylase (cytosine C5 methylase), its corresponding Hae III endonuclease, and λ DNA as their substrate.

Original languageEnglish
Pages (from-to)25-27
Number of pages3
JournalNatural Product Letters
Volume16
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

DNA-Cytosine Methylases
cytosine
Cytosine
Screening
Physiological Phenomena
screening
Genomic Imprinting
X Chromosome Inactivation
Deoxyribonuclease I
DNA
DNA Methylation
Carcinogenesis
genomic imprinting
X chromosome
DNA methylation
Chromosomes
Gene Expression
Gene expression
carcinogenesis
Assays

Keywords

  • DNA methylase
  • S-Adenosyl-L-methionine
  • Sinefungin

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

An in vitro screening method for DNA cytosine-C5-Methylase inhibitor. / Tamura, Takashi; Kataoka, Atsushi; Shu, Li Yin; Ashida, Akihiko; Tanaka, Hidehiko; Inagaki, Kenji.

In: Natural Product Letters, Vol. 16, No. 1, 2002, p. 25-27.

Research output: Contribution to journalArticle

Tamura, Takashi ; Kataoka, Atsushi ; Shu, Li Yin ; Ashida, Akihiko ; Tanaka, Hidehiko ; Inagaki, Kenji. / An in vitro screening method for DNA cytosine-C5-Methylase inhibitor. In: Natural Product Letters. 2002 ; Vol. 16, No. 1. pp. 25-27.
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