An extracellular serine protease produced by Vibrio vulnificus NCIMB 2137, a metalloprotease-gene negative strain isolated from a diseased eel

Shin ichi Miyoshi, Jiyou Wang, Keizo Katoh, Mitsutoshi Senoh, Tamaki Mizuno, Yoko Maehara

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Vibrio vulnificus is a ubiquitous estuarine microorganism but causes fatal systemic infections in immunocompromised humans, cultured eels or shrimps. An extracellular metalloprotease VVP/VvpE has been reported to be a potential virulence factor of the bacterium; however, a few strains isolated from a diseased eel or shrimp were recently found to produce a serine protease termed VvsA, but not VVP/VvpE. In the present study, we found that these strains had lost the 80 kb genomic region including the gene encoding VVP/VvpE. We also purified VvsA from the culture supernatant through ammonium sulfate fractionation, gel filtration and ion-exchange column chromatography, and the enzyme was demonstrated to be a chymotrypsin-like protease, as well as those from some vibrios. The gene vvsA was shown to constitute an operon with a downstream gene vvsB, and several Vibrio species were found to have orthologues of vvsAB. These findings indicate that the genes vvp/vvpE and vvsAB might be mobile genetic elements.

Original languageEnglish
Pages (from-to)1633-1639
Number of pages7
JournalWorld Journal of Microbiology and Biotechnology
Volume28
Issue number4
DOIs
Publication statusPublished - Apr 2012

Keywords

  • Metalloprotease
  • Polymerase chain reaction
  • Purification
  • Serine protease
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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