An exocellular thermolysin-like metalloprotease produced by Vibrio fluvialis

Purification, characterization, and gene cloning

Shin-ichi Miyoshi, Yuka Sonoda, Hiroko Wakiyama, Md Monzur Rahman, Ken Ichi Tomochika, Sumio Shinoda, Shigeo Yamamoto, Kazuo Tobe

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

An exocellular metalloprotease produced by Vibrio fluvialis, an enteropathogenic vibrio, was purified and characterized. The metalloprotease (V. fluvialis protease [VFP]) was found to have very similar characteristics to V. vulnificus protease, including a molecular mass of 45 kDa, sensitivity to chelating agents or competitive inhibitors for thermolysin-like metalloproteases, and the substrate specificity. The structural gene for VFP was also cloned, and its nucleotide sequence was determined. The deduced amino acid sequence confirmed that VFP was a member of the thermolysin family. VFP, like V. vulnificus protease, showed the haemagglutinating, permeability-enhancing and haemorrhagic activities in addition to the proteolytic activity toward oligopeptide, casein or elastin.

Original languageEnglish
Pages (from-to)127-134
Number of pages8
JournalMicrobial Pathogenesis
Volume33
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Thermolysin
Vibrio
Metalloproteases
Organism Cloning
Genes
Oligopeptides
Elastin
Chelating Agents
Substrate Specificity
Caseins
Amino Acid Sequence
Permeability
Peptide Hydrolases
protease V

Keywords

  • Haemagglutinin
  • Metalloprotease
  • Protease
  • Thermolysin
  • Vibrio fluvialis

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

An exocellular thermolysin-like metalloprotease produced by Vibrio fluvialis : Purification, characterization, and gene cloning. / Miyoshi, Shin-ichi; Sonoda, Yuka; Wakiyama, Hiroko; Rahman, Md Monzur; Tomochika, Ken Ichi; Shinoda, Sumio; Yamamoto, Shigeo; Tobe, Kazuo.

In: Microbial Pathogenesis, Vol. 33, No. 3, 2002, p. 127-134.

Research output: Contribution to journalArticle

Miyoshi, Shin-ichi ; Sonoda, Yuka ; Wakiyama, Hiroko ; Rahman, Md Monzur ; Tomochika, Ken Ichi ; Shinoda, Sumio ; Yamamoto, Shigeo ; Tobe, Kazuo. / An exocellular thermolysin-like metalloprotease produced by Vibrio fluvialis : Purification, characterization, and gene cloning. In: Microbial Pathogenesis. 2002 ; Vol. 33, No. 3. pp. 127-134.
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