An evolutionary 'intermediate state' of mitochondrial translation systems found in Trichinella species of parasitic nematodes

Co-evolution of tRNA and EF-Tu

Masashi Arita, Takuma Suematsu, Arihiro Osanai, Takashi Inaba, Haruo Kamiya, Kiyoshi Kita, Masahiko Sisido, Yoh Ichi Watanabe, Takashi Ohtsuki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

EF-Tu delivers aminoacyl-tRNAs to ribosomes in the translation system. However, unusual truncations found in some animal mitochondrial tRNAs seem to prevent recognition by a canonical EF-Tu. We showed previously that the chromadorean nematode has two distinct EF-Tus, one of which (EF-Tu1) binds only to T-armless aminoacyl-tRNAs and the other (EF-Tu2) binds to D-armless Ser-tRNAs. Neither of the EF-Tus can bind to canonical cloverleaf tRNAs. In this study, by analyzing the translation system of enoplean nematode Trichinella species, we address how EF-Tus and tRNAs have evolved from the canonical structures toward those of the chromadorean translation system. Trichinella mitochondria possess three types of tRNAs: cloverleaf tRNAs, which do not exist in chromadorean nematode mitochondria; T-armless tRNAs; and D-armless tRNAs. We found two mitochondrial EF-Tu species, EF-Tu1 and EF-Tu2, in Trichinella britovi. T.britovi EF-Tu2 could bind to only D-armless Ser-tRNA, as Caenorhabditis elegans EF-Tu2 does. In contrast to the case of C.elegans EF-Tu1, however, T.britovi EF-Tu1 bound to all three types of tRNA present in Trichinella mitochondria. These results suggest that Trichinella mitochondrial translation system, and particularly the tRNA-binding specificity of EF-Tu1, could be an intermediate state between the canonical system and the chromadorean nematode mitochondrial system.

Original languageEnglish
Pages (from-to)5291-5299
Number of pages9
JournalNucleic acids research.
Volume34
Issue number18
DOIs
Publication statusPublished - Oct 15 2006

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Trichinella
Peptide Elongation Factor Tu
Transfer RNA
RNA, Transfer, Ser
Mitochondria
Caenorhabditis elegans
Ribosomes

ASJC Scopus subject areas

  • Genetics

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An evolutionary 'intermediate state' of mitochondrial translation systems found in Trichinella species of parasitic nematodes : Co-evolution of tRNA and EF-Tu. / Arita, Masashi; Suematsu, Takuma; Osanai, Arihiro; Inaba, Takashi; Kamiya, Haruo; Kita, Kiyoshi; Sisido, Masahiko; Watanabe, Yoh Ichi; Ohtsuki, Takashi.

In: Nucleic acids research., Vol. 34, No. 18, 15.10.2006, p. 5291-5299.

Research output: Contribution to journalArticle

Arita, Masashi ; Suematsu, Takuma ; Osanai, Arihiro ; Inaba, Takashi ; Kamiya, Haruo ; Kita, Kiyoshi ; Sisido, Masahiko ; Watanabe, Yoh Ichi ; Ohtsuki, Takashi. / An evolutionary 'intermediate state' of mitochondrial translation systems found in Trichinella species of parasitic nematodes : Co-evolution of tRNA and EF-Tu. In: Nucleic acids research. 2006 ; Vol. 34, No. 18. pp. 5291-5299.
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