An enzyme immunoassay for human myoglobin and its application to forensic medicine

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have devised an enzyme immunoassay for the determination of human myoglobin. This assay provides superb reproducibility and a recovery rate without the influence of any anti-coagulants and hemolysis. The mean blood myoglobin level in healthy humans, determined by this assay, was 35.5 ± 23.9 ng/ml and the urine myoglobin level was found to be distributed over a broad range under 373.5 ng/ml. In cases of trauma sustained by living bodies, the blood myoglobin level was found to increase in accordance to the severity of trauma. Further, the myoglobin level in the blood began its increase during the early stage of the injury, and greater increases were shown in the elderly. In autopsy specimens, the ratio of the cardiac blood myoglobin level to the cerebrospinal fluid myoglobin level had a negative correlation with the postmortem passage of time and, as in the case of living bodies, the myoglobin level in the blood was considered a useful indicator of the severity of damage. However, since myoglobin level was found to vary, depending on the sites from where the sample blood was taken, the evaluation of the data should be done carefully. Finally, the simultaneous determination of the creatine phosphokinase (CPK) activity in autopsied blood samples would be of help for estimating the pestmortem passage of time and the progress of putrefaction.

Original languageEnglish
Pages (from-to)6-25
Number of pages20
JournalJapanese Journal of Legal Medicine
Volume45
Issue number1
Publication statusPublished - 1991

Fingerprint

Forensic Medicine
Myoglobin
Immunoenzyme Techniques
Wounds and Injuries
Coagulants
Creatine Kinase
Hemolysis
Cerebrospinal Fluid
Autopsy
Urine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

An enzyme immunoassay for human myoglobin and its application to forensic medicine. / Miyaishi, Satoru.

In: Japanese Journal of Legal Medicine, Vol. 45, No. 1, 1991, p. 6-25.

Research output: Contribution to journalArticle

@article{244de32ac41d443d936cba7832ec7106,
title = "An enzyme immunoassay for human myoglobin and its application to forensic medicine",
abstract = "We have devised an enzyme immunoassay for the determination of human myoglobin. This assay provides superb reproducibility and a recovery rate without the influence of any anti-coagulants and hemolysis. The mean blood myoglobin level in healthy humans, determined by this assay, was 35.5 ± 23.9 ng/ml and the urine myoglobin level was found to be distributed over a broad range under 373.5 ng/ml. In cases of trauma sustained by living bodies, the blood myoglobin level was found to increase in accordance to the severity of trauma. Further, the myoglobin level in the blood began its increase during the early stage of the injury, and greater increases were shown in the elderly. In autopsy specimens, the ratio of the cardiac blood myoglobin level to the cerebrospinal fluid myoglobin level had a negative correlation with the postmortem passage of time and, as in the case of living bodies, the myoglobin level in the blood was considered a useful indicator of the severity of damage. However, since myoglobin level was found to vary, depending on the sites from where the sample blood was taken, the evaluation of the data should be done carefully. Finally, the simultaneous determination of the creatine phosphokinase (CPK) activity in autopsied blood samples would be of help for estimating the pestmortem passage of time and the progress of putrefaction.",
author = "Satoru Miyaishi",
year = "1991",
language = "English",
volume = "45",
pages = "6--25",
journal = "Japanese Journal of Legal Medicine",
issn = "0047-1887",
publisher = "Medico-Legal Society of Japan",
number = "1",

}

TY - JOUR

T1 - An enzyme immunoassay for human myoglobin and its application to forensic medicine

AU - Miyaishi, Satoru

PY - 1991

Y1 - 1991

N2 - We have devised an enzyme immunoassay for the determination of human myoglobin. This assay provides superb reproducibility and a recovery rate without the influence of any anti-coagulants and hemolysis. The mean blood myoglobin level in healthy humans, determined by this assay, was 35.5 ± 23.9 ng/ml and the urine myoglobin level was found to be distributed over a broad range under 373.5 ng/ml. In cases of trauma sustained by living bodies, the blood myoglobin level was found to increase in accordance to the severity of trauma. Further, the myoglobin level in the blood began its increase during the early stage of the injury, and greater increases were shown in the elderly. In autopsy specimens, the ratio of the cardiac blood myoglobin level to the cerebrospinal fluid myoglobin level had a negative correlation with the postmortem passage of time and, as in the case of living bodies, the myoglobin level in the blood was considered a useful indicator of the severity of damage. However, since myoglobin level was found to vary, depending on the sites from where the sample blood was taken, the evaluation of the data should be done carefully. Finally, the simultaneous determination of the creatine phosphokinase (CPK) activity in autopsied blood samples would be of help for estimating the pestmortem passage of time and the progress of putrefaction.

AB - We have devised an enzyme immunoassay for the determination of human myoglobin. This assay provides superb reproducibility and a recovery rate without the influence of any anti-coagulants and hemolysis. The mean blood myoglobin level in healthy humans, determined by this assay, was 35.5 ± 23.9 ng/ml and the urine myoglobin level was found to be distributed over a broad range under 373.5 ng/ml. In cases of trauma sustained by living bodies, the blood myoglobin level was found to increase in accordance to the severity of trauma. Further, the myoglobin level in the blood began its increase during the early stage of the injury, and greater increases were shown in the elderly. In autopsy specimens, the ratio of the cardiac blood myoglobin level to the cerebrospinal fluid myoglobin level had a negative correlation with the postmortem passage of time and, as in the case of living bodies, the myoglobin level in the blood was considered a useful indicator of the severity of damage. However, since myoglobin level was found to vary, depending on the sites from where the sample blood was taken, the evaluation of the data should be done carefully. Finally, the simultaneous determination of the creatine phosphokinase (CPK) activity in autopsied blood samples would be of help for estimating the pestmortem passage of time and the progress of putrefaction.

UR - http://www.scopus.com/inward/record.url?scp=0025810883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025810883&partnerID=8YFLogxK

M3 - Article

C2 - 2046176

AN - SCOPUS:0025810883

VL - 45

SP - 6

EP - 25

JO - Japanese Journal of Legal Medicine

JF - Japanese Journal of Legal Medicine

SN - 0047-1887

IS - 1

ER -