An efflux transporter of silicon in rice

Jian Feng Ma, Naoki Yamaji, Namiki Mitani, Kazunori Tamai, Saeko Konishi, Toru Fujiwara, Maki Katsuhara, Masahiro Yano

Research output: Contribution to journalArticle

395 Citations (Scopus)

Abstract

Silicon is an important nutrient for the optimal growth and sustainable production of rice. Rice accumulates up to 10% silicon in the shoot, and this high accumulation is required to protect the plant from multiple abiotic and biotic stresses. A gene, Lsi1, that encodes a silicon influx transporter has been identified in rice. Here we describe a previously uncharacterized gene, low silicon rice 2 (Lsi2), which has no similarity to Lsi1. This gene is constitutively expressed in the roots. The protein encoded by this gene is localized, like Lsi1, on the plasma membrane of cells in both the exodermis and the endodermis, but in contrast to Lsi1, which is localized on the distal side, Lsi2 is localized on the proximal side of the same cells. Expression of Lsi2 in Xenopus oocytes did not result in influx transport activity for silicon, but preloading of the oocytes with silicon resulted in a release of silicon, indicating that Lsi2 is a silicon efflux transporter. The identification of this silicon transporter revealed a unique mechanism of nutrient transport in plants: having an influx transporter on one side and an efflux transporter on the other side of the cell to permit the effective transcellular transport of the nutrients.

Original languageEnglish
Pages (from-to)209-212
Number of pages4
JournalNature
Volume448
Issue number7150
DOIs
Publication statusPublished - Jul 12 2007

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Silicon
Food
Oocytes
Oryza
Genes
Transcytosis
Xenopus
Plasma Cells
Cell Membrane

ASJC Scopus subject areas

  • General

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Ma, J. F., Yamaji, N., Mitani, N., Tamai, K., Konishi, S., Fujiwara, T., ... Yano, M. (2007). An efflux transporter of silicon in rice. Nature, 448(7150), 209-212. https://doi.org/10.1038/nature05964

An efflux transporter of silicon in rice. / Ma, Jian Feng; Yamaji, Naoki; Mitani, Namiki; Tamai, Kazunori; Konishi, Saeko; Fujiwara, Toru; Katsuhara, Maki; Yano, Masahiro.

In: Nature, Vol. 448, No. 7150, 12.07.2007, p. 209-212.

Research output: Contribution to journalArticle

Ma, JF, Yamaji, N, Mitani, N, Tamai, K, Konishi, S, Fujiwara, T, Katsuhara, M & Yano, M 2007, 'An efflux transporter of silicon in rice', Nature, vol. 448, no. 7150, pp. 209-212. https://doi.org/10.1038/nature05964
Ma JF, Yamaji N, Mitani N, Tamai K, Konishi S, Fujiwara T et al. An efflux transporter of silicon in rice. Nature. 2007 Jul 12;448(7150):209-212. https://doi.org/10.1038/nature05964
Ma, Jian Feng ; Yamaji, Naoki ; Mitani, Namiki ; Tamai, Kazunori ; Konishi, Saeko ; Fujiwara, Toru ; Katsuhara, Maki ; Yano, Masahiro. / An efflux transporter of silicon in rice. In: Nature. 2007 ; Vol. 448, No. 7150. pp. 209-212.
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