An EAR-dependent regulatory module promotes male germ cell division and sperm fertility in Arabidopsis

Michael Borg, Nicholas Rutley, Sateesh Kagale, Yuki Hamamura, Mihai Gherghinoiu, Sanjeev Kumar, Ugur Sari, Manuel A. Esparza-Franco, Wataru Sakamoto, Kevin Rozwadowski, Tetsuya Higashiyama, David Twell

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The production of the sperm cells in angiosperms requires coordination of cell division and cell differentiation. In Arabidopsis thaliana, the germline-specific MYB protein DUO1 integrates these processes, but the regulatory hierarchy in which DUO1 functions is unknown. Here, we identify an essential role for two germline-specific DUO1 target genes, DAZ1 and DAZ2, which encode EAR motif-containing C2H2-type zinc finger proteins. We show that DAZ1/DAZ2 are required for germ cell division and for the proper accumulation of mitotic cyclins. Importantly, DAZ1/DAZ2 are sufficient to promote G2- to M-phase transition and germ cell division in the absence of DUO1. DAZ1/DAZ2 are also required for DUO1-dependent cell differentiation and are essential for gamete fusion at fertilization. We demonstrate that the two EAR motifs in DAZ1/DAZ2 mediate their function in the male germline and are required for transcriptional repression and for physical interaction with the corepressor TOPLESS. Our findings uncover an essential module in a regulatory hierarchy that drives mitotic transition in male germ cells and implicates gene repression pathways in sperm cell formation and fertility.

Original languageEnglish
Pages (from-to)2098-2113
Number of pages16
JournalPlant Cell
Volume26
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Arabidopsis
Germ Cells
Cell Division
Fertility
Spermatozoa
cell division
germ cells
spermatozoa
Cell Differentiation
Angiosperms
Co-Repressor Proteins
Cyclins
Phase Transition
cell differentiation
Fertilization
Genes
Proteins
zinc finger motif
cyclins
phase transition

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Borg, M., Rutley, N., Kagale, S., Hamamura, Y., Gherghinoiu, M., Kumar, S., ... Twell, D. (2014). An EAR-dependent regulatory module promotes male germ cell division and sperm fertility in Arabidopsis. Plant Cell, 26(5), 2098-2113. https://doi.org/10.1105/tpc.114.124743

An EAR-dependent regulatory module promotes male germ cell division and sperm fertility in Arabidopsis. / Borg, Michael; Rutley, Nicholas; Kagale, Sateesh; Hamamura, Yuki; Gherghinoiu, Mihai; Kumar, Sanjeev; Sari, Ugur; Esparza-Franco, Manuel A.; Sakamoto, Wataru; Rozwadowski, Kevin; Higashiyama, Tetsuya; Twell, David.

In: Plant Cell, Vol. 26, No. 5, 2014, p. 2098-2113.

Research output: Contribution to journalArticle

Borg, M, Rutley, N, Kagale, S, Hamamura, Y, Gherghinoiu, M, Kumar, S, Sari, U, Esparza-Franco, MA, Sakamoto, W, Rozwadowski, K, Higashiyama, T & Twell, D 2014, 'An EAR-dependent regulatory module promotes male germ cell division and sperm fertility in Arabidopsis', Plant Cell, vol. 26, no. 5, pp. 2098-2113. https://doi.org/10.1105/tpc.114.124743
Borg, Michael ; Rutley, Nicholas ; Kagale, Sateesh ; Hamamura, Yuki ; Gherghinoiu, Mihai ; Kumar, Sanjeev ; Sari, Ugur ; Esparza-Franco, Manuel A. ; Sakamoto, Wataru ; Rozwadowski, Kevin ; Higashiyama, Tetsuya ; Twell, David. / An EAR-dependent regulatory module promotes male germ cell division and sperm fertility in Arabidopsis. In: Plant Cell. 2014 ; Vol. 26, No. 5. pp. 2098-2113.
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