An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice

Guoxiong Chen, Takao Komatsuda, Jian Feng Ma, Christiane Nawrath, Mohammad Pourkheirandish, Akemi Tagiri, Yin Gang Hu, Mohammad Sameri, Xinrong Li, Xin Zhao, Yubing Liu, Chao Li, Xiaoying Ma, Aidong Wang, Sudha Nair, Ning Wang, Akio Miyao, Shun Sakuma, Naoki Yamaji, Xiuting ZhengEviatar Nevo

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88 Citations (Scopus)

Abstract

Land plants have developed a cuticle preventing uncontrolled water loss. Here we report that an ATP-binding cassette (ABC) subfamily G (ABCG) full transporter is required for leaf water conservation in both wild barley and rice. A spontaneous mutation, eibi1.b, in wild barley has a low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. Map-based cloning revealed that Eibi1 encodes an HvABCG31 full transporter. The gene was highly expressed in the elongation zone of a growing leaf (the site of cutin synthesis), and its gene product also was localized in developing, but not in mature tissue. A de novo wild barley mutant named "eibi1.c," along with two transposon insertion lines of rice mutated in the ortholog of HvABCG31 also were unable to restrict water loss from detached leaves. HvABCG31 is hypothesized to function as a transporter involved in cutin formation. Homologs of HvABCG31 were found in green algae, moss, and lycopods, indicating that this full transporter is highly conserved in the evolution of land plants.

Original languageEnglish
Pages (from-to)12354-12359
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number30
DOIs
Publication statusPublished - Jul 26 2011

Keywords

  • Cuticular wax
  • Drought resistance
  • Pleiotropic drug resistance
  • Radiation mutagenesis
  • Transposon Oryza sativa 17

ASJC Scopus subject areas

  • General

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    Chen, G., Komatsuda, T., Ma, J. F., Nawrath, C., Pourkheirandish, M., Tagiri, A., Hu, Y. G., Sameri, M., Li, X., Zhao, X., Liu, Y., Li, C., Ma, X., Wang, A., Nair, S., Wang, N., Miyao, A., Sakuma, S., Yamaji, N., ... Nevo, E. (2011). An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice. Proceedings of the National Academy of Sciences of the United States of America, 108(30), 12354-12359. https://doi.org/10.1073/pnas.1108444108