An artificial lipid bilayer formed on a PEG-coated glass for simultaneous electrical and optical measurement of single ion-channels

Toru Ide, Yuko Takeuchi, Takaaki Aoki, Kazuhito Tabata, Hiroyuki Noji

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The purpose of this study is to develop an apparatus for simultaneous measurement of electrical and spectroscopic parameters of single ion-channels. We have combined the single channel recording apparatus with an artificial lipid bilayer and a fluorescence microscope designed to detect single fluorescent molecules. The artificial membranes were formed on a PEG (polyethylene glycol)-coated glass and observed with an objective-type total internal reflection fluorescence (TIRF) microscope. The lateral motion of a single lipid molecule (β-BODIPY 530/550 HPC) was recorded. The lateral diffusion constant of the lipid molecule was calculated from the trajectories of single molecules as D = 8.0 ± 4.0 × 10-8 cm2/s. Ionic channels were incorporated into the membrane and single-channel current fluctuations were recorded. These data show the possibility of the present technique for simultaneous measurement of electrical and spectroscopic parameters of single-channel activities

Original languageEnglish
Journale-Journal of Surface Science and Nanotechnology
Volume3
DOIs
Publication statusPublished - Feb 25 2005
Externally publishedYes

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Keywords

  • Biological molecules -proteins
  • Biophysics, medical physics, biomedical engineering
  • BK-channel
  • Ion channel
  • Single molecule imaging

ASJC Scopus subject areas

  • Materials Science (miscellaneous)
  • Surfaces, Coatings and Films

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