An artificial lipid bilayer formed on a PEG-coated glass for simultaneous electrical and optical measurement of single ion-channels

Toru Ide, Yuko Takeuchi, Takaaki Aoki, Kazuhito Tabata, Hiroyuki Noji

Research output: Contribution to journalArticle

Abstract

The purpose of this study is to develop an apparatus for simultaneous measurement of electrical and spectroscopic parameters of single ion-channels. We have combined the single channel recording apparatus with an artificial lipid bilayer and a fluorescence microscope designed to detect single fluorescent molecules. The artificial membranes were formed on a PEG (polyethylene glycol)-coated glass and observed with an objective-type total internal reflection fluorescence (TIRF) microscope. The lateral motion of a single lipid molecule (β-BODIPY 530/550 HPC) was recorded. The lateral diffusion constant of the lipid molecule was calculated from the trajectories of single molecules as D = 8.0 ± 4.0 × 10-8 cm2/s. Ionic channels were incorporated into the membrane and single-channel current fluctuations were recorded. These data show the possibility of the present technique for simultaneous measurement of electrical and spectroscopic parameters of single-channel activities

Original languageEnglish
Journale-Journal of Surface Science and Nanotechnology
Volume3
DOIs
Publication statusPublished - Feb 25 2005
Externally publishedYes

Fingerprint

Lipid bilayers
Lipid Bilayers
Ion Channels
optical measurement
electrical measurement
Polyethylene glycols
Glass
lipids
glycols
polyethylenes
Molecules
glass
Ions
Fluorescence
Lipids
Artificial Membranes
molecules
Microscopes
microscopes
membranes

Keywords

  • Biological molecules -proteins
  • Biophysics, medical physics, biomedical engineering
  • BK-channel
  • Ion channel
  • Single molecule imaging

ASJC Scopus subject areas

  • Materials Science (miscellaneous)
  • Surfaces, Coatings and Films

Cite this

An artificial lipid bilayer formed on a PEG-coated glass for simultaneous electrical and optical measurement of single ion-channels. / Ide, Toru; Takeuchi, Yuko; Aoki, Takaaki; Tabata, Kazuhito; Noji, Hiroyuki.

In: e-Journal of Surface Science and Nanotechnology, Vol. 3, 25.02.2005.

Research output: Contribution to journalArticle

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AU - Noji, Hiroyuki

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