An approach to estimate radioadaptation from DSB repair efficiency

Fumio Yatagai, Kaoru Sugasawa, Shuichi Enomoto, Masamitsu Honma

Research output: Contribution to journalReview articlepeer-review

7 Citations (Scopus)

Abstract

In this review, we would like to introduce a unique approach for the estimation of radioadaptation. Recently, we proposed a new methodology for evaluating the repair efficiency of DNA double-strand breaks (DSB) using a model system. The model system can trace the fate of a single DSB, which is introduced within intron 4 of the TK gene on chromosome 17 in human lymphoblastoid TK6 cells by the expression of restriction enzyme I-SceI. This methodology was first applied to examine whether repair of the DSB (at the I-SceI site) can be influenced by low-dose, low-dose rate gamma-ray irradiation. We found that such low-dose IR exposure could enhance the activity of DSB repair through homologous recombination (HR). HR activity was also enhanced due to the pre-IR irradiation under the established conditions for radioadaptation (50 mGy X-ray-6 h-I-SceI treatment). Therefore, radioadaptation might account for the reduced frequency of homozygous loss of heterozygosity (LOH) events observed in our previous experiment (50 mGy X-ray-6 h-2 Gy X-ray). We suggest that the present evaluation of DSB repair using this I-SceI system, may contribute to our overall understanding of radioadaptation.

Original languageEnglish
Pages (from-to)407-413
Number of pages7
JournalJournal of radiation research
Volume50
Issue number5
DOIs
Publication statusPublished - 2009

Keywords

  • DSB repair
  • I-SceI digestion
  • Radioadaptation
  • TK6 cells

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

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