An antioxidant, N,N'-diphenyl-p-phenylenediamine (DPPD), affects labor and delivery in rats: A 28-day repeated dose test and reproduction/developmental toxicity test

Mariko Matsumoto, Makiko Yamaguchi, Yuka Yoshida, Mika Senuma, Hiromasa Takashima, Tomoko Kawamura, Hina Kato, Mika Takahashi, Mutsuko Hirata-Koizumi, Atsushi Ono, Kazuhito Yokoyama, Akihiko Hirose

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

A 28-day repeated dose toxicity test and reproduction/developmental toxicity test for N,N'-diphenyl-p-phenylenediamine (DPPD) were conducted in [Crl:CD(SD)] SPF rats. Male and female rats were dosed with DPPD by gavage for 28. days at 0, 100, 300, or 1000. mg/kg bw/day or for a total of 42-46. days at 0, 8, 50, or 300. mg/kg bw/day. No significant adverse effects were observed in the repeated dose toxicity study up to 1000. mg/kg bw/day in both sexes. In the reproduction/developmental toxicity study, two females showed piloerection, hypothermia, and pale skin; one died and the other showed dystocia on day 23 of pregnancy at 300. mg/kg bw/day. Another female delivered only three live pups at 300. mg/kg bw/day. A significantly prolonged gestation period was observed at 50 and 300. mg/kg bw/day. The NOAELs of repeated dose toxicity and reproduction/developmental toxicity were considered to be 1000 and 8. mg/kg bw/day, respectively.

Original languageEnglish
Pages (from-to)290-296
Number of pages7
JournalFood and Chemical Toxicology
Volume56
DOIs
Publication statusPublished - Jun 2013
Externally publishedYes

Keywords

  • Gestation period
  • N,N'-diphenyl-p-phenylenediamine
  • Prostaglandin
  • Repeated dose toxicity
  • Reproductive toxicity

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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