TY - JOUR
T1 - An anion channel from transverse tubular membranes incorporated into planar bilayers
AU - Ide, Toru
AU - Hidaka, Jun
AU - Kasai, Michiki
N1 - Funding Information:
The authors would like to thank Dr. Donald W. Landry for providing us with IAA. This work is supported in part by a Grant-in-Aid for Scientific Research to M.K. (04454620) and to T.I. (06858071) from the Ministry of Education, Science and Culture of Japan.
PY - 1995/7/26
Y1 - 1995/7/26
N2 - Transverse tubular (TT) vesicles from rabbit skeletal muscle were incorporated into planar lipid bilayers to characterize the chloride channel. The single channel conductance of the channel was 40 pS in choline-Cl solution (cis, 300 mM/ 100 mM, trans). The gating rate of the channel does not depend on membrane voltage. The channel was blocked by stilbene derivatives (DIDS and SITS), which are known as inhibitors of voltage-dependent Cl- channels of the Torpedo electric organ, from both sides of the membrane. An inhibitor of voltage-dependent Cl- channels of skeletal muscles, 9-anthracene carboxylic acid (9-AC) inhibited the channel from the cis side of the membranes, which corresponded to the cytoplasmic space. Ethacrynic acid (EA), which is reported to inhibit Cl- conductance of the kidney and trachea, decreased the open probability of the TT Cl- channel concentration dependently. Indanyloxyacetic acid (IAA), which is also reported to be an inhibitor of kidney and trachea Cl- channels, decreased the single channel current without affecting open probability of the TT Cl- channel.
AB - Transverse tubular (TT) vesicles from rabbit skeletal muscle were incorporated into planar lipid bilayers to characterize the chloride channel. The single channel conductance of the channel was 40 pS in choline-Cl solution (cis, 300 mM/ 100 mM, trans). The gating rate of the channel does not depend on membrane voltage. The channel was blocked by stilbene derivatives (DIDS and SITS), which are known as inhibitors of voltage-dependent Cl- channels of the Torpedo electric organ, from both sides of the membrane. An inhibitor of voltage-dependent Cl- channels of skeletal muscles, 9-anthracene carboxylic acid (9-AC) inhibited the channel from the cis side of the membranes, which corresponded to the cytoplasmic space. Ethacrynic acid (EA), which is reported to inhibit Cl- conductance of the kidney and trachea, decreased the open probability of the TT Cl- channel concentration dependently. Indanyloxyacetic acid (IAA), which is also reported to be an inhibitor of kidney and trachea Cl- channels, decreased the single channel current without affecting open probability of the TT Cl- channel.
KW - Anion channel
KW - Planar bilayer
KW - Transverse tubule
UR - http://www.scopus.com/inward/record.url?scp=0029151292&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029151292&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(95)00091-G
DO - 10.1016/0005-2736(95)00091-G
M3 - Article
C2 - 7632703
AN - SCOPUS:0029151292
VL - 1237
SP - 115
EP - 120
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 2
ER -