An animal model for human EBV-associated hemophagocytic syndrome: Herpesvirus papio frequently induces fatal lymphoproliferative disorders with hemophagocytic syndrome in rabbits

K. Hayashi, N. Ohara, N. Teramoto, S. Onoda, H. L. Chen, Takashi Oka, E. Kondo, Tadashi Yoshino, K. Takahashi, J. Yates, T. Akagi

Research output: Contribution to journalArticle

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Abstract

Epstein-Barr virus-associated hemophagocytic syndrome (EBV-AHS) is often associated with fatal infectious mononucleosis. However, the animal model for EBV-AHS has not been developed. We reported the first animal model for EBV-AHS using rabbits infected with EBV-related herpesvirus of baboon (HVP). Eleven of 13 (85%) rabbits inoculated intravenously with HVP-producing cells developed fatal lymphopro-liferative disorders (LPD) between 22 and 105 days after inoculation. LPD was also accompanied by hemophagocytic syndrome (HPS) in nine of these 11 rabbits. The peroral spray of cell-free HVP induced the virus infection with increased anti-EBV-viral capsid antigen-IgG titers in three of five rabbits, and two of these three infected rabbits died of LPD with HPS. Autopsy revealed hepatosplenomegaly and swollen lymph nodes. Atypical lymphoid T cells expressing EBV-encoded small RNA-1 infiltrated diffusely in many organs, frequently involving the lymph nodes, spleen, and liver. Hemophagocytic histiocytosis was observed in the lymph nodes, spleen, bone marrow, and thymus. HVP-DNA was detected in the tissues and peripheral blood from the infected rabbits by polymerase chain reaction or Southern blot analysis. Reverse transcriptase-polymerase chain reaction revealed both HVP-EBNA1 and HVP-EBNA2 transcripts, suggesting latency type III infection. These data indicate that the high rate of rabbit LPD with HPS induction is caused by HVP. This system is useful for studying the pathogenesis, prevention, and treatment of human EBV-AHS.

Original languageEnglish
Pages (from-to)1533-1542
Number of pages10
JournalAmerican Journal of Pathology
Volume158
Issue number4
Publication statusPublished - 2001

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Hemophagocytic Lymphohistiocytosis
Lymphoproliferative Disorders
Papio
Herpesviridae
Human Herpesvirus 4
Animal Models
Rabbits
Lymph Nodes
Spleen
Histiocytosis
Infectious Mononucleosis
Viral Antigens
Virus Diseases
Southern Blotting
Reverse Transcriptase Polymerase Chain Reaction
Thymus Gland
Autopsy
Immunoglobulin G
Bone Marrow
Lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

An animal model for human EBV-associated hemophagocytic syndrome : Herpesvirus papio frequently induces fatal lymphoproliferative disorders with hemophagocytic syndrome in rabbits. / Hayashi, K.; Ohara, N.; Teramoto, N.; Onoda, S.; Chen, H. L.; Oka, Takashi; Kondo, E.; Yoshino, Tadashi; Takahashi, K.; Yates, J.; Akagi, T.

In: American Journal of Pathology, Vol. 158, No. 4, 2001, p. 1533-1542.

Research output: Contribution to journalArticle

Hayashi, K. ; Ohara, N. ; Teramoto, N. ; Onoda, S. ; Chen, H. L. ; Oka, Takashi ; Kondo, E. ; Yoshino, Tadashi ; Takahashi, K. ; Yates, J. ; Akagi, T. / An animal model for human EBV-associated hemophagocytic syndrome : Herpesvirus papio frequently induces fatal lymphoproliferative disorders with hemophagocytic syndrome in rabbits. In: American Journal of Pathology. 2001 ; Vol. 158, No. 4. pp. 1533-1542.
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AU - Teramoto, N.

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AU - Chen, H. L.

AU - Oka, Takashi

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AU - Akagi, T.

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