An analysis of pathological activities of CCN proteins in joint disorders: Mechanical stretch-mediated CCN2 expression in cultured meniscus cells

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The multifunctional growth factor CYR61/CTGF/NOV (CCN) 2, also known as connective tissue growth factor, regulates cellular proliferation, differentiation, and tissue regeneration. Recent literatures have described important roles of CCN2 in the meniscus metabolism. However, the mechanical stressmediated transcriptional regulation of CCN2 in the meniscus remains unclear. The meniscus is a fibrocartilaginous tissue that controls complex biomechanics of the knee joint. Therefore, the injured unstable meniscus has a poor healing potential especially in the avascular inner region. In addition, dysfunction of the meniscus correlates with the progression of degenerative knee joint disorders and joint space narrowing. Here, we describe an experimental approach that investigates the distinct cellular behavior of inner and outer meniscus cells in response to mechanical stretch. Our experimental model can analyze the relationships between stretch-induced CCN2 expression and its functional role in the meniscus homeostasis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages533-542
Number of pages10
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)1064-3745

Fingerprint

Cultured Cells
Joints
Proteins
Knee Joint
Connective Tissue Growth Factor
Meniscus
Biomechanical Phenomena
Regeneration
Intercellular Signaling Peptides and Proteins
Homeostasis
Theoretical Models
Cell Proliferation

Keywords

  • CCN2
  • Mechanical stretch
  • Meniscus
  • Meniscus cells
  • Transcriptional regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

An analysis of pathological activities of CCN proteins in joint disorders : Mechanical stretch-mediated CCN2 expression in cultured meniscus cells. / Furumatsu, Takayuki; Ozaki, Toshihumi.

Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. p. 533-542 (Methods in Molecular Biology; Vol. 1489).

Research output: Chapter in Book/Report/Conference proceedingChapter

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