An amphotericin B-ergosterol covalent conjugate with powerful membrane permeabilizing activity

Nobuaki Matsumori, Noritsugu Eiraku, Shigeru Matsuoka, Tohru Oishi, Michio Murata, Takaaki Aoki, Toru Ide

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Amphotericin B-sterol conjugates were synthesized and examined for their membrane permeabilizing activity. Ergosterol and cholesterol, each connected with amphotericin B via an ethylenecarbamate or hexamethylenecarbamate linker, were examined by K+ flux assays using liposomes and by single-channel recording across phospholipid membrane. Among four conjugates tested, AmB-ergosterol bearing an ethylenecarbamate linker exhibited the most powerful activity, which substantially exceeded that of the cholesterol homolog. Single-channel recording clearly exhibited that the ergosterol conjugate elicited channel current with the conductance of 28 pS, which was comparable with those by AmB, and revealed a higher channel open probability than the cholesterol conjugate. These results imply that direct interaction between amphotericin B and ergosterol is reproduced by their conjugate, which may serve as a model compound for understanding the drug's selective toxicity.

Original languageEnglish
Pages (from-to)673-679
Number of pages7
JournalChemistry and Biology
Volume11
Issue number5
DOIs
Publication statusPublished - May 2004
Externally publishedYes

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Ergosterol
Amphotericin B
Membranes
Cholesterol
Bearings (structural)
Sterols
Drug-Related Side Effects and Adverse Reactions
Liposomes
Toxicity
Assays
Phospholipids
Fluxes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

An amphotericin B-ergosterol covalent conjugate with powerful membrane permeabilizing activity. / Matsumori, Nobuaki; Eiraku, Noritsugu; Matsuoka, Shigeru; Oishi, Tohru; Murata, Michio; Aoki, Takaaki; Ide, Toru.

In: Chemistry and Biology, Vol. 11, No. 5, 05.2004, p. 673-679.

Research output: Contribution to journalArticle

Matsumori, Nobuaki ; Eiraku, Noritsugu ; Matsuoka, Shigeru ; Oishi, Tohru ; Murata, Michio ; Aoki, Takaaki ; Ide, Toru. / An amphotericin B-ergosterol covalent conjugate with powerful membrane permeabilizing activity. In: Chemistry and Biology. 2004 ; Vol. 11, No. 5. pp. 673-679.
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