TY - JOUR
T1 - An additional limb can be induced from the flank of the chick embryo by FGF4
AU - Ohuchi, Hideyo
AU - Nakagawa, Takashi
AU - Yamauchi, Masaki
AU - Ohata, Takeshi
AU - Yoshioka, Hidefumi
AU - Kuwana, Takashi
AU - Mima, Tatsuo
AU - Mikawa, Takashi
AU - Nohno, Tsutumu
AU - Noji, Sumihare
PY - 1995/4/26
Y1 - 1995/4/26
N2 - To elucidate what initiates formation of the limb, we have attempted to induce an additional limb from the flank of the chick embryo by infecting retrovirus or implanting cells. We report here that an additional limb can be formed from the flank when we implant fibroblast growth factor 4 (Fgf4)-expressing cells into the lateral plate mesoderm at the pre-limb bud stage. In a newly formed limb bud, expressions of both Sonic hedgehog and chick Fgf4, which are authentic morphogenetic signals from the zone of polarizing activity and the apical ectodermal ridge, respectively, are induced by the implanted cells. Thus, it is concluded that the competence for limb development is present along the flank of the chick embryo and that FGF4 applied ectopically at the pre-limb bud stage can alter the developmental fate of flank cells to become limb cells. The present experimental system will contribute to a further elucidation on how the limb is formed.
AB - To elucidate what initiates formation of the limb, we have attempted to induce an additional limb from the flank of the chick embryo by infecting retrovirus or implanting cells. We report here that an additional limb can be formed from the flank when we implant fibroblast growth factor 4 (Fgf4)-expressing cells into the lateral plate mesoderm at the pre-limb bud stage. In a newly formed limb bud, expressions of both Sonic hedgehog and chick Fgf4, which are authentic morphogenetic signals from the zone of polarizing activity and the apical ectodermal ridge, respectively, are induced by the implanted cells. Thus, it is concluded that the competence for limb development is present along the flank of the chick embryo and that FGF4 applied ectopically at the pre-limb bud stage can alter the developmental fate of flank cells to become limb cells. The present experimental system will contribute to a further elucidation on how the limb is formed.
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U2 - 10.1006/bbrc.1995.1572
DO - 10.1006/bbrc.1995.1572
M3 - Article
C2 - 7733973
AN - SCOPUS:0029068414
VL - 209
SP - 809
EP - 816
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -