An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy

Yuya Tomonaga, Tetsurou Hidaka, Izuru Kawamura, Takudo Nishio, Kazuhiro Ohsawa, Takashi Okitsu, Akimori Wada, Yuki Sudo, Naoki Kamo, Ayyalusamy Ramamoorthy, Akira Naito

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Abstract

A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The 13C NMR signals from [20- 13C]retinal- ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuously photoirradiated condition. NMR signals observed from the photoactivated retinal provide insights into the process of photocycle in the ppR/pHtrII complex.

Original languageEnglish
JournalBiophysical Journal
Volume101
Issue number10
DOIs
Publication statusPublished - Nov 16 2011
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics

Cite this

Tomonaga, Y., Hidaka, T., Kawamura, I., Nishio, T., Ohsawa, K., Okitsu, T., Wada, A., Sudo, Y., Kamo, N., Ramamoorthy, A., & Naito, A. (2011). An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy. Biophysical Journal, 101(10). https://doi.org/10.1016/j.bpj.2011.10.022