TY - JOUR
T1 - An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy
AU - Tomonaga, Yuya
AU - Hidaka, Tetsurou
AU - Kawamura, Izuru
AU - Nishio, Takudo
AU - Ohsawa, Kazuhiro
AU - Okitsu, Takashi
AU - Wada, Akimori
AU - Sudo, Yuki
AU - Kamo, Naoki
AU - Ramamoorthy, Ayyalusamy
AU - Naito, Akira
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research on Priority Area (21017002) and a Grant-in-Aid for Young Scientists (B) (22770101) from the Ministry of Culture, Sports, Science and Technology of Japan and a SUNBOR Grant from Suntory Institute for Bioorganic Research. A.R. was a fellow of the Japan Society for the Promotion of Science during this research in Japan. This research was partly supported by the National Institutes of Health (GM084018 and GM095640 to A.R.).
PY - 2011/11/16
Y1 - 2011/11/16
N2 - A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The 13C NMR signals from [20- 13C]retinal- ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuously photoirradiated condition. NMR signals observed from the photoactivated retinal provide insights into the process of photocycle in the ppR/pHtrII complex.
AB - A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The 13C NMR signals from [20- 13C]retinal- ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuously photoirradiated condition. NMR signals observed from the photoactivated retinal provide insights into the process of photocycle in the ppR/pHtrII complex.
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U2 - 10.1016/j.bpj.2011.10.022
DO - 10.1016/j.bpj.2011.10.022
M3 - Article
C2 - 22098758
AN - SCOPUS:81255164760
VL - 101
SP - L50-L52
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 10
ER -