An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy

Yuya Tomonaga; Tetsurou Hidaka; Izuru Kawamura; Takudo Nishio; Kazuhiro Ohsawa; Takashi Okitsu; Akimori Wada; Yuki Sudo; Naoki Kamo; Ayyalusamy Ramamoorthy; Akira Naito

doi:10.1016/j.bpj.2011.10.022

An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy

Yuya Tomonaga, Tetsurou Hidaka, Izuru Kawamura, Takudo Nishio, Kazuhiro Ohsawa, Takashi Okitsu, Akimori Wada, Yuki Sudo, Naoki Kamo, Ayyalusamy Ramamoorthy, Akira Naito

Research output: Contribution to journal › Article

18 Citations (Scopus)

Abstract

A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The ¹³C NMR signals from [20- ¹³C]retinal- ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuously photoirradiated condition. NMR signals observed from the photoactivated retinal provide insights into the process of photocycle in the ppR/pHtrII complex.

Original language	English
Pages (from-to)	L50-L52
Journal	Biophysical Journal
Volume	101
Issue number	10
DOIs	https://doi.org/10.1016/j.bpj.2011.10.022
Publication status	Published - Nov 16 2011
Externally published	Yes

ASJC Scopus subject areas

Biophysics

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Tomonaga, Y., Hidaka, T., Kawamura, I., Nishio, T., Ohsawa, K., Okitsu, T., Wada, A., Sudo, Y., Kamo, N., Ramamoorthy, A., & Naito, A. (2011). An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy. Biophysical Journal, 101(10), L50-L52. https://doi.org/10.1016/j.bpj.2011.10.022

An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy. / Tomonaga, Yuya; Hidaka, Tetsurou; Kawamura, Izuru; Nishio, Takudo; Ohsawa, Kazuhiro; Okitsu, Takashi; Wada, Akimori; Sudo, Yuki; Kamo, Naoki; Ramamoorthy, Ayyalusamy; Naito, Akira.

In: Biophysical Journal, Vol. 101, No. 10, 16.11.2011, p. L50-L52.

Research output: Contribution to journal › Article

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AU - Wada, Akimori

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