An active DNA transposon nDart causing leaf variegation and mutable dwarfism and its related elements in rice

Kazuo Tsugane, Masahiko Maekawa, Kyoko Takagi, Hiroyuki Takahara, Qian Qian, Chang Ho Eun, Shigeru Iida

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

While characterized mutable alleles caused by DNA transposons have been abundant in maize since the discovery of Dissociation conferring variegation by Barbara McClintock, only a few mutable alleles have been described in rice even though the rice genome contains various transposons. Here, we show that a spontaneous mutable virescent allele, pyl-v, is caused by the disruption of the nuclear-coded essential chloroplast protease gene, OsClpP5, due to insertion of a 607-bp non-autonomous DNA transposon, non-autonomous DNA-based active rice transposon one (nDart1), belonging to the hAT superfamily. The transposition of nDart1 can be induced by crossing with a line containing an autonomous element, aDart, and stabilized by segregating out of aDart. We also identified a novel mutable dwarf allele thl-m caused by an insertion of nDart1. The japonica cultivar Nipponbare carries no aDart, although it contains epigenetically silenced Dart element(s), which can be activated by 5-azacytidine. Nipponbare bears four subgroups of about 3.6-kb Dart-like sequences, three of which contain potential transposase genes, and around 3.6-kb elements without an apparent transposase gene, as well as three subgroups of about 0.6-kb nDart1-related elements that are all internal deletions of the Dart-like sequences. Both nDart1q1 and 3.6-kb Dart-like elements were also present in indica varieties 93-11 and Kasalath. nDart1 appears to be the most active mutagen among nDart1-related elements contributing to generating natural variations. A candidate for an autonomous element, aDart, and a possible application of nDart1 for transposon tagging are discussed.

Original languageEnglish
Pages (from-to)46-57
Number of pages12
JournalPlant Journal
Volume45
Issue number1
DOIs
Publication statusPublished - Jan 2006

Fingerprint

Dwarfism
DNA Transposable Elements
dwarfing
transposons
Alleles
Transposases
rice
alleles
DNA
leaves
Chloroplast Genes
Azacitidine
Essential Genes
Mutagens
Barbara
genes
transposition (genetics)
Genes
Zea mays
Peptide Hydrolases

Keywords

  • Clp gene
  • DNA transposon
  • Dwarf mutation
  • Gene tagging
  • Mutable allele
  • Rice genome

ASJC Scopus subject areas

  • Plant Science

Cite this

An active DNA transposon nDart causing leaf variegation and mutable dwarfism and its related elements in rice. / Tsugane, Kazuo; Maekawa, Masahiko; Takagi, Kyoko; Takahara, Hiroyuki; Qian, Qian; Eun, Chang Ho; Iida, Shigeru.

In: Plant Journal, Vol. 45, No. 1, 01.2006, p. 46-57.

Research output: Contribution to journalArticle

Tsugane, K, Maekawa, M, Takagi, K, Takahara, H, Qian, Q, Eun, CH & Iida, S 2006, 'An active DNA transposon nDart causing leaf variegation and mutable dwarfism and its related elements in rice', Plant Journal, vol. 45, no. 1, pp. 46-57. https://doi.org/10.1111/j.1365-313X.2005.02600.x
Tsugane, Kazuo ; Maekawa, Masahiko ; Takagi, Kyoko ; Takahara, Hiroyuki ; Qian, Qian ; Eun, Chang Ho ; Iida, Shigeru. / An active DNA transposon nDart causing leaf variegation and mutable dwarfism and its related elements in rice. In: Plant Journal. 2006 ; Vol. 45, No. 1. pp. 46-57.
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