Amyloplast membrane protein SUBSTANDARD STARCH GRAIN6 controls starch grain size in rice endosperm

Ryo Matsushima, Masahiko Maekawa, Miyako Kusano, Katsura Tomita, Hideki Kondo, Hideki Nishimura, Naoko Crofts, Naoko Fujita, Wataru Sakamoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Starch is a biologically and commercially important polymer of glucose. Starch is organized into starch grains (SGs) inside amyloplasts. The SG size differs depending on the plant species and is one of the most important factors for industrial applications of starch. There is limited information on genetic factors regulating SG sizes. In this study, we report the rice (Oryza sativa) mutant substandard starch grain6 (ssg6), which develops enlarged SGs in endosperm. Enlarged SGs are observed starting at 3 d after flowering. During endosperm development, a number of smaller SGs appear and coexist with enlarged SGs in the same cells. The ssg6 mutation also affects SG morphologies in pollen. The SSG6 gene was identified by map-based cloning and microarray analysis. SSG6 encodes a protein homologous to aminotransferase. SSG6 differs from other rice homologs in that it has a transmembrane domain. SSG6-green fluorescent protein is localized in the amyloplast membrane surrounding SGs in rice endosperm, pollen, and pericarp. The results of this study suggest that SSG6 is a novel protein that controls SG size. SSG6 will be a useful molecular tool for future starch breeding and applications.

Original languageEnglish
Pages (from-to)1445-1459
Number of pages15
JournalPlant Physiology
Volume170
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

amyloplasts
Endosperm
Plastids
starch granules
membrane proteins
Starch
endosperm
Membrane Proteins
rice
starch
Oryza
pollen
Pollen
industrial applications
transaminases
pericarp
green fluorescent protein
molecular cloning
Oryza sativa
polymers

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Amyloplast membrane protein SUBSTANDARD STARCH GRAIN6 controls starch grain size in rice endosperm. / Matsushima, Ryo; Maekawa, Masahiko; Kusano, Miyako; Tomita, Katsura; Kondo, Hideki; Nishimura, Hideki; Crofts, Naoko; Fujita, Naoko; Sakamoto, Wataru.

In: Plant Physiology, Vol. 170, No. 3, 01.03.2016, p. 1445-1459.

Research output: Contribution to journalArticle

Matsushima, Ryo ; Maekawa, Masahiko ; Kusano, Miyako ; Tomita, Katsura ; Kondo, Hideki ; Nishimura, Hideki ; Crofts, Naoko ; Fujita, Naoko ; Sakamoto, Wataru. / Amyloplast membrane protein SUBSTANDARD STARCH GRAIN6 controls starch grain size in rice endosperm. In: Plant Physiology. 2016 ; Vol. 170, No. 3. pp. 1445-1459.
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