Amyloplast membrane protein SUBSTANDARD STARCH GRAIN6 controls starch grain size in rice endosperm

Ryo Matsushima, Masahiko Maekawa, Miyako Kusano, Katsura Tomita, Hideki Kondo, Hideki Nishimura, Naoko Crofts, Naoko Fujita, Wataru Sakamoto

    Research output: Contribution to journalArticlepeer-review

    30 Citations (Scopus)

    Abstract

    Starch is a biologically and commercially important polymer of glucose. Starch is organized into starch grains (SGs) inside amyloplasts. The SG size differs depending on the plant species and is one of the most important factors for industrial applications of starch. There is limited information on genetic factors regulating SG sizes. In this study, we report the rice (Oryza sativa) mutant substandard starch grain6 (ssg6), which develops enlarged SGs in endosperm. Enlarged SGs are observed starting at 3 d after flowering. During endosperm development, a number of smaller SGs appear and coexist with enlarged SGs in the same cells. The ssg6 mutation also affects SG morphologies in pollen. The SSG6 gene was identified by map-based cloning and microarray analysis. SSG6 encodes a protein homologous to aminotransferase. SSG6 differs from other rice homologs in that it has a transmembrane domain. SSG6-green fluorescent protein is localized in the amyloplast membrane surrounding SGs in rice endosperm, pollen, and pericarp. The results of this study suggest that SSG6 is a novel protein that controls SG size. SSG6 will be a useful molecular tool for future starch breeding and applications.

    Original languageEnglish
    Pages (from-to)1445-1459
    Number of pages15
    JournalPlant physiology
    Volume170
    Issue number3
    DOIs
    Publication statusPublished - Mar 2016

    ASJC Scopus subject areas

    • Physiology
    • Genetics
    • Plant Science

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