Amurubicinol-induced eotaxin-3 expression in human NCI-H69 small cell lung carcinoma cells

Masami Niiya, Kenji Niiya, Misako Shibakura, Noboru Asaumi, Chikamasa Yoshida, Mitsune Tanimoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We previously demonstrated the doxorubicin-induced expression of urokinase-type plasminogen activator (uPA), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α in human RCK8 lymphoma cells and NCI-H69 small cell lung carcinoma cells in which reactive oxygen species might be involved. Amurubicin hydrochloride (AMR), a novel derivative drug of doxorubicin, was recently introduced to clinical practice for treatment of lung cancer in Japan. Therefore, we investigated the effects of AMR on the expression of uPA and chemokines in NCI-H69 cells. AMR and its active form, amurubicinol hydrochloride (AMROH), both induced the expression of uPA, IL-8 and MCP-1 in H69 cells in a dose-dependent manner. When the cultured supernatant obtained from AMRtreated H69 cells was subcutaneously injected into rabbits, migration of a significant number of eosinophils was observed around the injected site. Antigen levels of eotaxin-3, a major migration-factor of eosinophils, were increased in AMROH-treated cells in parallel with the mRNA levels. The induction was observed below the clinically achievable concentration of AMR or AMROH. Thus, the simultaneous induction of uPA, IL-8, MCP-1 and eotaxin-3 may play a role in the pharmacological action of AMR through induction of the interaction between proinflammatory cells and lung carcinoma cells.

Original languageEnglish
Pages (from-to)571-576
Number of pages6
JournalOncology reports
Volume15
Issue number3
Publication statusPublished - Mar 1 2006

Keywords

  • Amurubicin
  • Amurubicinol
  • Eotaxin-3
  • Interleukin-8
  • Monocyte chemoattractant protein-1
  • Small cell lung carcinoma cells
  • Urokinase-type plasminogen activator

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Niiya, M., Niiya, K., Shibakura, M., Asaumi, N., Yoshida, C., & Tanimoto, M. (2006). Amurubicinol-induced eotaxin-3 expression in human NCI-H69 small cell lung carcinoma cells. Oncology reports, 15(3), 571-576.