Amplification of MYCL in atypical ewing tumor. analysis of metaphase and microarray comparative genomic hybridization

Toshihumi Ozaki, Yasuko Nakagawa, Aki Yoshida, Kunihiko Numoto, Shinnsuke Sugihara, Toshiyuki Kunisada, Shuji Hamazaki, Hajime Inoue

Research output: Contribution to journalArticle

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Abstract

A 20-year-old man developed a soft tissue mass in his right upper arm and, 3 months later, was referred to our hospital. The tumor cells showed brisk mitotic activity and a large amount of cytoplasmic glycogen was demonstrated with periodic acid Schiff stain. A diagnosis of atypical Ewing sarcoma was made. Chemotherapy according to the VACA protocol, comprising vincristine, actinomycin D, cyclophosphamide and doxorubicin was started. The chemotherapy was effective and a limb salvage procedure was performed by implantation of an autoclaved bone after wide tumor excision. During the postoperative chemotherapy, a local recurrence and multiple metastases developed, and the patient died due to disease progression. Fourteen years later, this tumor sample, preserved in a deepfreeze, was analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) to detect the fusion gene. This tumor had an EWS exon 7 to FLI1 exon 6 fusion transcript. Moreover, metaphase and microarray comparative genomic hybridization (CGH) was done to detect chromosomal instabilities. Many gains and losses were noted on metaphase CGH, and MYCL amplification was identified on microarray CGH.

Original languageEnglish
Pages (from-to)275-282
Number of pages8
JournalCancer Genomics and Proteomics
Volume1
Issue number4
Publication statusPublished - Jul 2004

Fingerprint

Ewing's Sarcoma
Comparative Genomic Hybridization
Metaphase
Microarray Analysis
Microarrays
Chemotherapy
Amplification
Tumors
Drug Therapy
Exons
Neoplasms
Fusion reactions
Salvaging
Chromosomal Instability
Limb Salvage
Periodic Acid
Gene Fusion
Polymerase chain reaction
RNA-Directed DNA Polymerase
Dactinomycin

Keywords

  • Comparative genomic hybidization
  • Ewing tumor
  • Microarray

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cancer Research
  • Biochemistry

Cite this

Amplification of MYCL in atypical ewing tumor. analysis of metaphase and microarray comparative genomic hybridization. / Ozaki, Toshihumi; Nakagawa, Yasuko; Yoshida, Aki; Numoto, Kunihiko; Sugihara, Shinnsuke; Kunisada, Toshiyuki; Hamazaki, Shuji; Inoue, Hajime.

In: Cancer Genomics and Proteomics, Vol. 1, No. 4, 07.2004, p. 275-282.

Research output: Contribution to journalArticle

Ozaki, T, Nakagawa, Y, Yoshida, A, Numoto, K, Sugihara, S, Kunisada, T, Hamazaki, S & Inoue, H 2004, 'Amplification of MYCL in atypical ewing tumor. analysis of metaphase and microarray comparative genomic hybridization', Cancer Genomics and Proteomics, vol. 1, no. 4, pp. 275-282.
Ozaki, Toshihumi ; Nakagawa, Yasuko ; Yoshida, Aki ; Numoto, Kunihiko ; Sugihara, Shinnsuke ; Kunisada, Toshiyuki ; Hamazaki, Shuji ; Inoue, Hajime. / Amplification of MYCL in atypical ewing tumor. analysis of metaphase and microarray comparative genomic hybridization. In: Cancer Genomics and Proteomics. 2004 ; Vol. 1, No. 4. pp. 275-282.
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