Amphiphysin I and regulation of synaptic vesicle endocytosis

Yumei Wu, Hideki Matsui, Kazuhito Tomizawa

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Amphiphysin I, known as a major dynamin-binding partner localized on the collars of nascent vesicles, plays a key role in clathrin-mediated endocytosis (CME) of synaptic vesicles. Amphiphysin I mediates the invagination and fission steps of synaptic vesicles by sensing or facilitating membrane curvature and stimulating the GTPase activity of dynamin. Amphiphysin I may form a homodimer by itself or a heterodimer with amphiphysin II in vivo. Both amphiphysin I and II function as multilinker proteins in the clathrin-coated complex. Under normal physiological conditions, the functions of amphiphysin I and some other endocytic proteins are known to be regulated by phosphorylation and dephosphorylation. During hyperexcited conditions, the most recent data showed that amphiphysin I is truncated by the ca2 -dependent protease calpain. Overexpression of the truncated form of amphiphysin I inhibited transferrin uptake and synaptic vesicle endocytosis (SVE). This suggests that amphiphysin I may be an important regulator for SVE when massive amounts of Ca2+ flow into presynaptic terminals, a phenomenon observed in neurodegenerative disorders such as ischemia/anoxia, epilepsy, stroke, trauma and Alzheimer's disease. This review describes current knowledge regarding the general properties and functions of amphiphysin I as well as the functional regulations such as phosphorylation and proteolysis in nerve terminals.

Original languageEnglish
Pages (from-to)305-323
Number of pages19
JournalActa Medica Okayama
Volume63
Issue number6
Publication statusPublished - Dec 2009

Fingerprint

Synaptic Vesicles
Endocytosis
Dynamins
Clathrin
Phosphorylation
amphiphysin
Proteolysis
Calpain
GTP Phosphohydrolases
Presynaptic Terminals
Transferrin
Neurodegenerative Diseases
Epilepsy
Alzheimer Disease
Proteins
Peptide Hydrolases
Ischemia
Stroke
Membranes

Keywords

  • Amphiphysin I
  • Calpain
  • Hyperexcitation
  • Seizure
  • SVE

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wu, Y., Matsui, H., & Tomizawa, K. (2009). Amphiphysin I and regulation of synaptic vesicle endocytosis. Acta Medica Okayama, 63(6), 305-323.

Amphiphysin I and regulation of synaptic vesicle endocytosis. / Wu, Yumei; Matsui, Hideki; Tomizawa, Kazuhito.

In: Acta Medica Okayama, Vol. 63, No. 6, 12.2009, p. 305-323.

Research output: Contribution to journalArticle

Wu, Y, Matsui, H & Tomizawa, K 2009, 'Amphiphysin I and regulation of synaptic vesicle endocytosis', Acta Medica Okayama, vol. 63, no. 6, pp. 305-323.
Wu Y, Matsui H, Tomizawa K. Amphiphysin I and regulation of synaptic vesicle endocytosis. Acta Medica Okayama. 2009 Dec;63(6):305-323.
Wu, Yumei ; Matsui, Hideki ; Tomizawa, Kazuhito. / Amphiphysin I and regulation of synaptic vesicle endocytosis. In: Acta Medica Okayama. 2009 ; Vol. 63, No. 6. pp. 305-323.
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