Aminopropyl-silicate membrane for microcapsule-shaped bioartificial organs: Control of molecular permeability

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A novel microcapsule membrane composed of aminopropyl-silicate was synthesized on Ca-alginate gel beads from 3-aminopropyltrimethoxysilane (APTrMOS) and tetramethoxysilane (TMOS) by the sol-gel method. Preparation conditions such as the molar ratio of APTrMOS to TMOS, the sequence of addition and the contact time of each precursor were examined to give the membrane immunoisolatable permeability for microcapsule-shaped bioartificial organs.The permeability of the membrane decreased with an increase of up to 2.4 in the molar ratio, with γ-globulin being completely rejected at this ratio. Addition of APTrMOS prior to TMOS and allowing 1min of contact with the Ca-alginate gel beads suspended in n-hexane was effective for rejection of γ-globulin. The optimized aminopropyl-silicate membrane was estimated to give a molecular weight cut-off point of less than 150kDa, as required for immunoisolation, and an excellent permeability to low molecular weight substances such as glucose and oxygen. Our data show that the permeability of the aminopropyl-silicate membrane, that is the microscopic structure of the membrane, is strongly controllable by altering preparation conditions.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalJournal of Membrane Science
Volume202
Issue number1-2
DOIs
Publication statusPublished - Jun 15 2002
Externally publishedYes

Keywords

  • Aminopropyl-silicate
  • Immunoisolation membrane
  • Microcapsule
  • Microcapsule-shaped bioartificial organs
  • Sol-gel method

ASJC Scopus subject areas

  • Biochemistry
  • Materials Science(all)
  • Physical and Theoretical Chemistry
  • Filtration and Separation

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